The experimental evidence for the realization of a superplastic behavior with 900% elongation in V-alloyed high-nitrogen austenitic Fe-19Cr-22Mn-1.5V-0.3C-0.6N steel was proposed. Using thermomechanical processing, a misoriented grain/subgrain austenitic microstructure with a high density of deformation-assisted defects and precipitates was developed in the steel. During high-temperature tensile deformation in a temperature interval from 850 to 1000 °C and strain-rate range from 4 × 10−4 s−1 to 6 × 10−3 s−1, this microstructure demonstrated the characteristics of superplastic flow: elongation in the interval 400−900%, strain-rate sensitivity exponent m = 0.40−0.49, grain boundary sliding mechanism. The maximum elongation to failure (900%) was reached at deformation temperature 950 °C and strain rate 4 × 10−4 s−1.