Nature Communications (Nov 2021)
A standardized gnotobiotic mouse model harboring a minimal 15-member mouse gut microbiota recapitulates SOPF/SPF phenotypes
- Marion Darnaud,
- Filipe De Vadder,
- Pascaline Bogeat,
- Lilia Boucinha,
- Anne-Laure Bulteau,
- Andrei Bunescu,
- Céline Couturier,
- Ana Delgado,
- Hélène Dugua,
- Céline Elie,
- Alban Mathieu,
- Tereza Novotná,
- Djomangan Adama Ouattara,
- Séverine Planel,
- Adrien Saliou,
- Dagmar Šrůtková,
- Jennifer Yansouni,
- Bärbel Stecher,
- Martin Schwarzer,
- François Leulier,
- Andrea Tamellini
Affiliations
- Marion Darnaud
- BIOASTER, Institut de Recherche Technologique
- Filipe De Vadder
- Institut de Génomique Fonctionnelle de Lyon, Université de Lyon, Ecole Normale Supérieure de Lyon, Centre National de la Recherche Scientifique, Université Claude Bernard Lyon 1
- Pascaline Bogeat
- BIOASTER, Institut de Recherche Technologique
- Lilia Boucinha
- BIOASTER, Institut de Recherche Technologique
- Anne-Laure Bulteau
- Institut de Génomique Fonctionnelle de Lyon, Université de Lyon, Ecole Normale Supérieure de Lyon, Centre National de la Recherche Scientifique, Université Claude Bernard Lyon 1
- Andrei Bunescu
- BIOASTER, Institut de Recherche Technologique
- Céline Couturier
- BIOASTER, Institut de Recherche Technologique
- Ana Delgado
- BIOASTER, Institut de Recherche Technologique
- Hélène Dugua
- BIOASTER, Institut de Recherche Technologique
- Céline Elie
- BIOASTER, Institut de Recherche Technologique
- Alban Mathieu
- BIOASTER, Institut de Recherche Technologique
- Tereza Novotná
- Laboratory of Gnotobiology, Institute of Microbiology of the Czech Academy of Sciences
- Djomangan Adama Ouattara
- BIOASTER, Institut de Recherche Technologique
- Séverine Planel
- BIOASTER, Institut de Recherche Technologique
- Adrien Saliou
- BIOASTER, Institut de Recherche Technologique
- Dagmar Šrůtková
- Laboratory of Gnotobiology, Institute of Microbiology of the Czech Academy of Sciences
- Jennifer Yansouni
- BIOASTER, Institut de Recherche Technologique
- Bärbel Stecher
- Max von Pettenkofer Institute of Hygiene and Medical Microbiology, Ludwig-Maximilians-University of Munich
- Martin Schwarzer
- Laboratory of Gnotobiology, Institute of Microbiology of the Czech Academy of Sciences
- François Leulier
- BIOASTER, Institut de Recherche Technologique
- Andrea Tamellini
- BIOASTER, Institut de Recherche Technologique
- DOI
- https://doi.org/10.1038/s41467-021-26963-9
- Journal volume & issue
-
Vol. 12,
no. 1
pp. 1 – 21
Abstract
Here, the authors develop and characterize a mouse microbiota composed of 15 strains representative of the intestinal microbiota found in C57BL/6J specific opportunistic- and pathogen-free (C57Bl/6J SOPF) mice and derive a new standardized gnotobiotic mouse model, called GM15, which recapitulates the phenotypes of SOPF or SPF animals in different animal facilities with improved reproducibility.
