Nature Communications (Nov 2021)

A standardized gnotobiotic mouse model harboring a minimal 15-member mouse gut microbiota recapitulates SOPF/SPF phenotypes

  • Marion Darnaud,
  • Filipe De Vadder,
  • Pascaline Bogeat,
  • Lilia Boucinha,
  • Anne-Laure Bulteau,
  • Andrei Bunescu,
  • Céline Couturier,
  • Ana Delgado,
  • Hélène Dugua,
  • Céline Elie,
  • Alban Mathieu,
  • Tereza Novotná,
  • Djomangan Adama Ouattara,
  • Séverine Planel,
  • Adrien Saliou,
  • Dagmar Šrůtková,
  • Jennifer Yansouni,
  • Bärbel Stecher,
  • Martin Schwarzer,
  • François Leulier,
  • Andrea Tamellini

DOI
https://doi.org/10.1038/s41467-021-26963-9
Journal volume & issue
Vol. 12, no. 1
pp. 1 – 21

Abstract

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Here, the authors develop and characterize a mouse microbiota composed of 15 strains representative of the intestinal microbiota found in C57BL/6J specific opportunistic- and pathogen-free (C57Bl/6J SOPF) mice and derive a new standardized gnotobiotic mouse model, called GM15, which recapitulates the phenotypes of SOPF or SPF animals in different animal facilities with improved reproducibility.