Serologic Hepatitis B Virus Immunity in Health Care Workers

Abstract

Health care workers (HCWs) are at high risk of blood borne infections including Hepatitis B virus (HBV) infection. HBV vaccination is recommended for HCWs but post vaccination testing of immune response (anti-HBs) is not routinely performed. In our study information on immune response after the first immunization schedule of HCWs is not available. By reason of the questions regarding long lasting immunity, we decided to assess the anti-HBs of HCWs who wished to check immune response after different times of vaccination and also unvaccinated persons in St. Marina University Hospital, Varna, Bulgaria. After informed consent, 341 HCWs were investigated. They were divided into 3 groups according to their status: Group A had no history of vaccination against HBV, Group B had no complete vaccination schedule and Group C had complete vaccination data. Of Group C, 32 had been vaccinated more than 10 years previously, 111 - 10–5 years previously and 48 - < 5 years previously. Quantitative detection of antibody to HBsAg (anti-HBs) by commercial ELISA was carried out. A total, positive immune response was detected in 35.6% (group A), 66.2% (group B) and 80.1% (group C) of HCWs investigated. Of Group C positive immune response was detected in 68.7%, 81.1% and 85.4% respectively of the time of vaccination. Detectable anti-HB was found in HCWs without HBV immunization, probably after unknown exposure to HBV. The lack of information regarding immune response after the first immunization schedule makes the interpretation of no detectible anti-HBs level 5–10 years post-immunization difficult. For the HCWs with anti-HBs loss, counseling for booster vaccine dose and consequent testing is mandatory.