Journal of Applied Oral Science (Oct 2009)

Cytotoxicity evaluation of two root canal sealers and a commercial calcium hydroxide paste on THP1 cell line by Trypan Blue assay

  • Gabriela Tavares Bekner Correa,
  • Gabriel Alves Costa Veranio,
  • Licínio Esmaeraldo Silva,
  • Raphael Hirata Junior,
  • Jeffry M. Coil,
  • Miriam F. Zaccaro Scelza

DOI
https://doi.org/10.1590/S1678-77572009000500020
Journal volume & issue
Vol. 17, no. 5
pp. 457 – 461

Abstract

Read online

OBJECTIVE: The aim of this investigation was to evaluate the cytotoxicity of two brands of root canal sealers, epoxy-resin based and zinc oxide-eugenol based, and one commercial calcium hydroxide paste on a monocyte cell line THP-1. MATERIAL AND METHODS: Undiluted (crude extract) and diluted extracts to 10%, 1%, 0.1%, 0.01%, 0.001% and 0.0001% of the sealers were tested for cytotoxicity to THP-1 cells using the trypan blue assay. Extracts were obtained according to ISO standard. Data were analyzed statistically by the Kruskal-Wallis and Mann-Whitney tests at 5% significance level. RESULTS: Crude extract of AH Plus and Fill Canal killed approximately 90% of THP-1 cells versus 36% of THP-1 cells killed by L&C crude extract (p0.05), except for L&C 1% extract. CONCLUSIONS: The results revealed that the L&C paste crude extract was less cytotoxic to THP-1 cells than AH Plus or Fill Canal crude extracts.

Keywords