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Cross-Linking Enhances Deposition of Human Endothelial Progenitor Cells in the Rat Heart after Intracoronary Transplantation

Cell Transplantation. 2010;19 DOI 10.3727/096368909X474834

 

Journal Homepage

Journal Title: Cell Transplantation

ISSN: 0963-6897 (Print); 1555-3892 (Online)

Publisher: SAGE Publishing

LCC Subject Category: Medicine

Country of publisher: United States

Language of fulltext: English

Full-text formats available: PDF, HTML

 

AUTHORS


Sandra Burghoff (Institute for Cardiovascular Physiology, Heinrich Heine University Duesseldorf, Duesseldorf, Germany)

Zhaoping Ding (Institute for Cardiovascular Physiology, Heinrich Heine University Duesseldorf, Duesseldorf, Germany)

Angelika Blaszczyk (Institute for Cardiovascular Physiology, Heinrich Heine University Duesseldorf, Duesseldorf, Germany)

Andreas Wirrwar (Clinic for Nuclear Medicine, Heinrich Heine University Duesseldorf, Duesseldorf, Germany)

Doris Buchholz (Clinic for Nuclear Medicine, Heinrich Heine University Duesseldorf, Duesseldorf, Germany)

Hans-Wilhelm Müller (Clinic for Nuclear Medicine, Heinrich Heine University Duesseldorf, Duesseldorf, Germany)

Jürgen Schrader (Institute for Cardiovascular Physiology, Heinrich Heine University Duesseldorf, Duesseldorf, Germany)

EDITORIAL INFORMATION

Blind peer review

Editorial Board

Instructions for authors

Time From Submission to Publication: 15 weeks

 

Abstract | Full Text

Transplantation of human endothelial progenitor cells (hEPCs) may improve vascularization and left ventricular function after myocardial infarction. The scope of this study was to explore, whether cross-linking of EPCs may enhance the deposition of cells in the rat heart after clinical-like, intracoronary transplantation. To this end, 111 In-oxinate-labeled hEPCs were infused by a minimally invasive technique into the coronary arteries of immunosuppressed Wistar rats under control conditions and after ischemia/reperfusion. In a second set of experiments hEPCs were treated with phytohemagglutinin to create small cell clusters prior to transplantation. Continous three-dimensional HiSPECT images for 1 h and after 48 h revealed that cell deposition was significantly higher when hEPCs were cross-linked. Therefore, cross-linking of hEPCs may provide a promising approach to enhance the number of trapped cells also in a clinical setting.