Frontiers in Immunology (Oct 2021)

A Dynamic Transcriptome Map of Different Tissue Microenvironment Cells Identified During Gastric Cancer Development Using Single-Cell RNA Sequencing

  • Honghao Yin,
  • Honghao Yin,
  • Honghao Yin,
  • Rui Guo,
  • Rui Guo,
  • Rui Guo,
  • Huanyu Zhang,
  • Huanyu Zhang,
  • Huanyu Zhang,
  • Songyi Liu,
  • Songyi Liu,
  • Songyi Liu,
  • Yuehua Gong,
  • Yuehua Gong,
  • Yuehua Gong,
  • Yuan Yuan,
  • Yuan Yuan,
  • Yuan Yuan

DOI
https://doi.org/10.3389/fimmu.2021.728169
Journal volume & issue
Vol. 12

Abstract

Read online

Gastric cancer (GC) development trends have identified multiple processes ranging from inflammation to carcinogenesis, however, key pathogenic mechanisms remain unclear. Tissue microenvironment (TME) cells are critical for the progression of malignant tumors. Here, we generated a dynamic transcriptome map of various TME cells during multi-disease stages using single-cell sequencing analysis. We observed a set of key transition markers related to TME cell carcinogenic evolution, and delineated landmark dynamic carcinogenic trajectories of these cells. Of these, macrophages, fibroblasts, and endothelial cells exerted considerable effects toward epithelial cells, suggesting these cells may be key TME factors promoting GC occurrence and development. Our results suggest a phenotypic convergence of different TME cell types toward tumor formation processes in GC. We believe our data would pave the way for early GC detection, diagnosis, and treatment therapies.

Keywords