Detection of Quorum Sensing Activity in the Multidrug-Resistant Clinical Isolate Pseudomonas aeruginosa Strain GB11

Abstract

A multidrug-resistant clinical bacteria strain GB11 was isolated from a wound swab on the leg of a patient. Identity of stain GB11 as Pseudomonas aeruginosa was validated by using matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry (MALDI-TOF MS). Detection of the production of signaling molecules, N-acylhomoserine lactones (AHLs), was conducted using three different bacterial biosensors. A total of four different AHLs were found to be produced by strain GB11, namely N-butyryl homoserine lactone (C4-HSL), N-hexanoylhomoserine lactone (C6-HSL), N-octanoyl homoserine lactone (C8-HSL) and N-3-oxo-dodecanoylhomoserine lactone (3-oxo-C12-HSL) using high resolution liquid chromatography tandem mass spectrometry (LC-MS/MS). Of these detected AHLs, 3-oxo-C12-HSL was found to be the most abundant AHL produced by P. aeruginosa GB11.

Keywords

• MALDI-TOF
• mass spectrometry
• N-butyryl homoserine lactone (C4-HSL)
• N-hexanoyl homoserine lactone (C6-HSL)
• N-octanoyl homoserine lactone (C8-HSL)
• N-dodecanoyl homoserine lactone (C12-HSL)
• N-3-oxo-dodecanoylhomoserine lactone (3-oxo-C12-HSL)
• clinical pathogen
• cell-to-cell communication