Generation of an induced pluripotent stem cell (iPSC) line from a diabetic patient with glucagon receptor (GCGR) p.W83X mutation

Yang Liu; Yang Liu; Yunbo Jia; Jing Lv; Dan Sun; Yalei Yin; Nan Liu; Benli Su; Guangwei Sun

Stem Cell Research (May 2022)

Generation of an induced pluripotent stem cell (iPSC) line from a diabetic patient with glucagon receptor (GCGR) p.W83X mutation

Yang Liu,
Yang Liu,
Yunbo Jia,
Jing Lv,
Dan Sun,
Yalei Yin,
Nan Liu,
Benli Su,
Guangwei Sun

Affiliations

Yang Liu: Innovative Engineering Technology Research Center for Cell Therapy, Shengjing Hospital of China Medical University, Shenyang 110022, China; Scientific Research Center for Translational Medicine, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China
Yang Liu: Scientific Research Center for Translational Medicine, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China; Laboratory of Cancer Metabolism, Shengjing Hospital of China Medical University, Shenyang 117004, China
Yunbo Jia: Innovative Engineering Technology Research Center for Cell Therapy, Shengjing Hospital of China Medical University, Shenyang 110022, China
Jing Lv: Scientific Research Center for Translational Medicine, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China
Dan Sun: Scientific Research Center for Translational Medicine, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China
Yalei Yin: Scientific Research Center for Translational Medicine, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China
Nan Liu: The Second Hospital of Dalian Medical University, Dalian 116023, China
Benli Su: The Second Hospital of Dalian Medical University, Dalian 116023, China; Corresponding authors at: The Second Hospital of Dalian Medical University, Dalian 116023, China (B. Su), Innovative Engineering Technology Research Center for Cell Therapy, Shengjing Hospital of China Medical University, Shenyang 110022, China (G. Sun).
Guangwei Sun: Innovative Engineering Technology Research Center for Cell Therapy, Shengjing Hospital of China Medical University, Shenyang 110022, China; Scientific Research Center for Translational Medicine, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China; Corresponding authors at: The Second Hospital of Dalian Medical University, Dalian 116023, China (B. Su), Innovative Engineering Technology Research Center for Cell Therapy, Shengjing Hospital of China Medical University, Shenyang 110022, China (G. Sun).

Journal volume & issue: Vol. 61
p. 102745

Abstract

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Induced pluripotent stem cell (iPSC) line, SJHi001-A, was generated from a diabetic patient carrying a heterozygous c.G248A mutation in GCGR gene that generated the p.W83X. The PBMCs from her father (no diabetes and no mutation site) were also prepared into iPSC (SJHi002-A) as a control. Both two cell lines had normal karyotype, expressed pluripotency markers and could differentiate into the three germ layers in vivo.

Published in Stem Cell Research

ISSN: 1873-5061 (Print); 1876-7753 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Science: Biology (General)
Website: https://www.journals.elsevier.com/stem-cell-research

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