Circulating Pro-Vascular Progenitor Cell Depletion During Type 2 Diabetes

Daniella C. Terenzi, BHSc; Mohammed Al-Omran, MD, MSc; Adrian Quan, MPhil; Hwee Teoh, PhD; Subodh Verma, MD, PhD; David A. Hess, PhD

JACC: Basic to Translational Science (Feb 2019)

Circulating Pro-Vascular Progenitor Cell Depletion During Type 2 Diabetes

Daniella C. Terenzi, BHSc,
Mohammed Al-Omran, MD, MSc,
Adrian Quan, MPhil,
Hwee Teoh, PhD,
Subodh Verma, MD, PhD,
David A. Hess, PhD

Affiliations

Daniella C. Terenzi, BHSc: Division of Cardiac Surgery, Keenan Research Centre for Biomedical Science and Li Ka Shing Knowledge Institute of St. Michael’s Hospital, Toronto, Ontario, Canada; Institute of Medical Science, University of Toronto, Toronto, Ontario, Canada
Mohammed Al-Omran, MD, MSc: Institute of Medical Science, University of Toronto, Toronto, Ontario, Canada; Division of Vascular Surgery, Keenan Research Centre for Biomedical Science and Li Ka Shing Knowledge Institute of St. Michael’s Hospital, Toronto, Ontario, Canada; Department of Surgery, University of Toronto, Toronto, Ontario, Canada; Department of Pharmacology and Toxicology, University of Toronto, Toronto, Ontario, Canada
Adrian Quan, MPhil: Division of Cardiac Surgery, Keenan Research Centre for Biomedical Science and Li Ka Shing Knowledge Institute of St. Michael’s Hospital, Toronto, Ontario, Canada
Hwee Teoh, PhD: Division of Cardiac Surgery, Keenan Research Centre for Biomedical Science and Li Ka Shing Knowledge Institute of St. Michael’s Hospital, Toronto, Ontario, Canada; Division of Endocrinology and Metabolism, Li Ka Shing Knowledge Institute of St. Michael’s Hospital, Toronto, Ontario, Canada
Subodh Verma, MD, PhD: Division of Cardiac Surgery, Keenan Research Centre for Biomedical Science and Li Ka Shing Knowledge Institute of St. Michael’s Hospital, Toronto, Ontario, Canada; Institute of Medical Science, University of Toronto, Toronto, Ontario, Canada; Department of Surgery, University of Toronto, Toronto, Ontario, Canada; Department of Pharmacology and Toxicology, University of Toronto, Toronto, Ontario, Canada; Dr. Subodh Verma, Division of Cardiac Surgery, St. Michael’s Hospital, Suite 8-003, 30 Bond Street, Toronto, Ontario M5B 1W8, Canada.
David A. Hess, PhD: Division of Vascular Surgery, Keenan Research Centre for Biomedical Science and Li Ka Shing Knowledge Institute of St. Michael’s Hospital, Toronto, Ontario, Canada; Department of Pharmacology and Toxicology, University of Toronto, Toronto, Ontario, Canada; Robarts Research Institute, London, Ontario, Canada; Address for correspondence: Dr. David A. Hess, Robarts Research Institute, Western University, 1153 Richmond Street, London, Ontario N6A 5B7, Canada.

Journal volume & issue: Vol. 4, no. 1
pp. 98 – 112

Abstract

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Summary: Detection of vascular regenerative cell exhaustion is required to combat ischemic complications during type 2 diabetes mellitus (T2D). We used high aldehyde dehydrogenase (ALDH) activity and surface marker co-expression to develop a high-throughput flow cytometry–based assay to quantify circulating proangiogenic and proinflammatory cell content in the peripheral blood of individuals with T2D. Circulating proangiogenic monocytes expressing anti-inflammatory M2 markers were decreased in patients with T2D. Individuals with longer duration of T2D exhibited reduced frequencies of circulating proangiogenic ALDHhiCD34+ progenitor cells with primitive (CD133) and migratory (CXCR4) phenotypes. This approach consistently detected increased inflammatory cell burden and decreased provascular progenitor content in individuals with T2D. Key Words: aldehyde dehydrogenase, angiogenesis, ischemia, progenitor cells, type 2 diabetes

Published in JACC: Basic to Translational Science

ISSN: 2452-302X (Online)
Publisher: Elsevier
Country of publisher: United States
LCC subjects: Medicine: Internal medicine: Specialties of internal medicine: Diseases of the circulatory (Cardiovascular) system
Website: https://www.journals.elsevier.com/jacc-basic-to-translational-science/

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