Cytotoxicity of dental cement on soft tissue associated with dental implants at different time intervals [version 2; peer review: 2 approved]
Ann Sales,
Vignesh Kamath,
Thilak Shetty B,
Umesh Y. Pai,
Sharon Saldanha,
Mahesh Mandatheje,
Puneeth Hegde,
Sandipan Mukherjee,
Shama Prasada Kabekkodu,
Akshar Bajaj,
Prashanth Bajantri,
Shobha J. Rodrigues
Affiliations
Ann Sales
Department of Prosthodontics and Crown and Bridge, Manipal College of Dental Sciences, Mangalore, Manipal Academy of Higher Education, Manipal, Karnataka, 576104, India
Department of Prosthodontics and Crown and Bridge, Manipal College of Dental Sciences, Mangalore, Manipal Academy of Higher Education, Manipal, Karnataka, 576104, India
Thilak Shetty B
Department of Prosthodontics and Crown and Bridge, Manipal College of Dental Sciences, Mangalore, Manipal Academy of Higher Education, Manipal, Karnataka, 576104, India
Department of Prosthodontics and Crown and Bridge, Manipal College of Dental Sciences, Mangalore, Manipal Academy of Higher Education, Manipal, Karnataka, 576104, India
Department of Prosthodontics and Crown and Bridge, Manipal College of Dental Sciences, Mangalore, Manipal Academy of Higher Education, Manipal, Karnataka, 576104, India
Mahesh Mandatheje
Department of Prosthodontics and Crown and Bridge, Manipal College of Dental Sciences, Mangalore, Manipal Academy of Higher Education, Manipal, Karnataka, 576104, India
Puneeth Hegde
Department of Prosthodontics and Crown and Bridge, Manipal College of Dental Sciences, Mangalore, Manipal Academy of Higher Education, Manipal, Karnataka, 576104, India
Sandipan Mukherjee
Department of Prosthodontics and Crown and Bridge, Manipal College of Dental Sciences, Mangalore, Manipal Academy of Higher Education, Manipal, Karnataka, 576104, India
Cell and Molecular Biology, Manipal Academy of Higher Education Manipal, Manipal, Karnataka, 576104, India
Akshar Bajaj
Department of Biomaterials and Restorative Sciences, Henry M Goldman School of Dental Medicine Boston University, United States Of America, United States Of America, USA
Department of Prosthodontics and Crown and Bridge, Manipal College of Dental Sciences, Mangalore, Manipal Academy of Higher Education, Manipal, Karnataka, 576104, India
Shobha J. Rodrigues
Department of Prosthodontics and Crown and Bridge, Manipal College of Dental Sciences, Mangalore, Manipal Academy of Higher Education, Manipal, Karnataka, 576104, India
Background To investigate and compare the effect of four commercially used dental cement at 24 hours, 48 hours,72 hours (h) and 6 days on the cellular response of human gingival fibroblast (HGF). Methods 3 cement pellet samples were made for each 4-test cement (n=12). The cement used for this study were zinc phosphate (ZP), zinc oxide non-eugenol (ZOE), RelyX U200 (RU200), and glass ionomer cement (GIC). The cytotoxicity of peri-implant tissues was investigated using one commercial cell line. All processing was done following International Organization for Standardization (ISO) methods 10993-5 and 10993-12 (MTT assay Test). Cell cultures without dental cement were considered as control. Standard laboratory procedures were followed to permit cell growth and confluence over 48 hrs after sub-cultivation. Before being subjected to analysis, the cells were kept in direct contact with the cement samples for the suggested time period. To validate the results the specimens were tested three times each. Cell death and inhibition of cell growth were measured quantitatively. Results were analyzed using 1-way ANOVA (a=0.05) followed by Tukey B post hoc test. Results The study showed the dental cement test material was cytotoxic. ZOE, ZP, GIC, and RU200 were cytotoxic in decreasing order, respectively, significantly reducing cell viability after exposure to HGF (p <0.001). Conclusions Within the limitations of this in-vitro cellular study, results indicated that HGF were vulnerable to the test the dental cement. The highest cytotoxicity was observed in ZOE, followed by ZP, GIC, and RU200.
