Frontiers in Cell and Developmental Biology (Nov 2023)

A novel chromatin-remodeling complex variant, dcPBAF, is involved in maintaining transcription in differentiated neurons

  • Nataliya V. Soshnikova,
  • Nataliya V. Soshnikova,
  • Nataliya V. Soshnikova,
  • Asya M. Azieva,
  • Asya M. Azieva,
  • Nataliya S. Klimenko,
  • Alvina I. Khamidullina,
  • Alexey V. Feoktistov,
  • Alexey V. Feoktistov,
  • Andrey A. Sheynov,
  • Alexander V. Brechalov,
  • Victor V. Tatarskiy,
  • Sofia G. Georgieva

DOI
https://doi.org/10.3389/fcell.2023.1271598
Journal volume & issue
Vol. 11

Abstract

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The Polybromo-associated BAF (BRG1- or BRM-associated factors) (PBAF) chromatin-remodeling complex is essential for transcription in mammalian cells. In this study, we describe a novel variant of the PBAF complex from differentiated neuronal cells, called dcPBAF, that differs from the canonical PBAF existing in proliferating neuroblasts. We describe that in differentiated adult neurons, a specific subunit of PBAF, PHF10, is replaced by a PHF10 isoform that lacks N- and C-terminal domains (called PHF10D). In addition, dcPBAF does not contain the canonical BRD7 subunit. dcPBAF binds promoters of the actively transcribed neuron-specific and housekeeping genes in terminally differentiated neurons of adult mice. Furthermore, in differentiated human neuronal cells, PHF10D-containing dcPBAF maintains a high transcriptional level at several neuron-specific genes.

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