Genetic Analysis and Fine Mapping of QTL for the Erect Leaf in Mutant <i>mths29</i> Induced through Fast Neutron in Wheat
Zhixin Yang,
Jiayu Gu,
Minghui Zhao,
Xiaofeng Fan,
Huijun Guo,
Yongdun Xie,
Jinfeng Zhang,
Hongchun Xiong,
Linshu Zhao,
Shirong Zhao,
Yuping Ding,
Fuquan Kong,
Li Sui,
Le Xu,
Luxiang Liu
Affiliations
Zhixin Yang
College of Agriculture, Yangtze University, Jingzhou 434023, China
Jiayu Gu
State Key Laboratory of Crop Gene Resources and Breeding, National Engineering Laboratory for Crop Molecular Breeding, National Center of Space Mutagenesis for Crop Improvement, CAEA Research and Development Center on Nuclear Technology Applications for Irradiation Mutation Breeding, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, China
Minghui Zhao
Dry-Land Farming Institute of Hebei Academy of Agricultural and Forestry Sciences, Hengshui 053000, China
Xiaofeng Fan
College of Agriculture, Yangtze University, Jingzhou 434023, China
Huijun Guo
State Key Laboratory of Crop Gene Resources and Breeding, National Engineering Laboratory for Crop Molecular Breeding, National Center of Space Mutagenesis for Crop Improvement, CAEA Research and Development Center on Nuclear Technology Applications for Irradiation Mutation Breeding, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, China
Yongdun Xie
State Key Laboratory of Crop Gene Resources and Breeding, National Engineering Laboratory for Crop Molecular Breeding, National Center of Space Mutagenesis for Crop Improvement, CAEA Research and Development Center on Nuclear Technology Applications for Irradiation Mutation Breeding, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, China
Jinfeng Zhang
State Key Laboratory of Crop Gene Resources and Breeding, National Engineering Laboratory for Crop Molecular Breeding, National Center of Space Mutagenesis for Crop Improvement, CAEA Research and Development Center on Nuclear Technology Applications for Irradiation Mutation Breeding, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, China
Hongchun Xiong
State Key Laboratory of Crop Gene Resources and Breeding, National Engineering Laboratory for Crop Molecular Breeding, National Center of Space Mutagenesis for Crop Improvement, CAEA Research and Development Center on Nuclear Technology Applications for Irradiation Mutation Breeding, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, China
Linshu Zhao
State Key Laboratory of Crop Gene Resources and Breeding, National Engineering Laboratory for Crop Molecular Breeding, National Center of Space Mutagenesis for Crop Improvement, CAEA Research and Development Center on Nuclear Technology Applications for Irradiation Mutation Breeding, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, China
Shirong Zhao
State Key Laboratory of Crop Gene Resources and Breeding, National Engineering Laboratory for Crop Molecular Breeding, National Center of Space Mutagenesis for Crop Improvement, CAEA Research and Development Center on Nuclear Technology Applications for Irradiation Mutation Breeding, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, China
Yuping Ding
State Key Laboratory of Crop Gene Resources and Breeding, National Engineering Laboratory for Crop Molecular Breeding, National Center of Space Mutagenesis for Crop Improvement, CAEA Research and Development Center on Nuclear Technology Applications for Irradiation Mutation Breeding, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, China
Fuquan Kong
China Institute of Atomic Energy, Beijing 102413, China
Li Sui
China Institute of Atomic Energy, Beijing 102413, China
Le Xu
College of Agriculture, Yangtze University, Jingzhou 434023, China
Luxiang Liu
State Key Laboratory of Crop Gene Resources and Breeding, National Engineering Laboratory for Crop Molecular Breeding, National Center of Space Mutagenesis for Crop Improvement, CAEA Research and Development Center on Nuclear Technology Applications for Irradiation Mutation Breeding, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, China
The erect leaf plays a crucial role in determining plant architecture, with its growth and development regulated by genetic factors. However, there has been a lack of comprehensive studies on the regulatory mechanisms governing wheat lamina joint development, thus failing to meet current breeding demands. In this study, a wheat erect leaf mutant, mths29, induced via fast neutron mutagenesis, was utilized for QTL fine mapping and investigation of lamina joint development. Genetic analysis of segregating populations derived from mths29 and Jimai22 revealed that the erect leaf trait was controlled by a dominant single gene. Using BSR sequencing and map-based cloning techniques, the QTL responsible for the erect leaf trait was mapped to a 1.03 Mb physical region on chromosome 5A. Transcriptome analysis highlighted differential expression of genes associated with cell division and proliferation, as well as several crucial transcription factors and kinases implicated in lamina joint development, particularly in the boundary cells of the preligule zone in mths29. These findings establish a solid foundation for understanding lamina joint development and hold promise for potential improvements in wheat plant architecture.
