Phenolic Characterization Using cLC-DAD Analysis and Evaluation of In Vitro and In Vivo Pharmacological Activities of <i>Ruta tuberculata</i> Forssk
Asma Saidi,
Leila Hambaba,
Mohamed Sabri Bensaad,
Mohamed Akram Melakhessou,
Chawki Bensouici,
Nouicer Ferhat,
Mohamed Amine Kahoul,
Mahmoud Helal,
Rokayya Sami,
Saif A. Alharthy,
Roua S. Baty,
Nouf H. Alsubhi,
Ghadeer I. Alrefaei,
Abeer Elhakem,
Sarah Alharthi,
Fahmy G. Elsaid,
Ali A. Shati
Affiliations
Asma Saidi
Laboratory of Biotechnology of Bioactive Molecules and Cellular Physiopathology (LBMBPC), Department of Microbiology and Biochemistry, Faculty of Natural and Life Sciences, University Batna 2, Batna 05078, Algeria
Leila Hambaba
Laboratory of Biotechnology of Bioactive Molecules and Cellular Physiopathology (LBMBPC), Department of Microbiology and Biochemistry, Faculty of Natural and Life Sciences, University Batna 2, Batna 05078, Algeria
Mohamed Sabri Bensaad
Laboratory of Biotechnology of Bioactive Molecules and Cellular Physiopathology (LBMBPC), Department of Microbiology and Biochemistry, Faculty of Natural and Life Sciences, University Batna 2, Batna 05078, Algeria
Mohamed Akram Melakhessou
Laboratory of Biotechnology of Bioactive Molecules and Cellular Physiopathology (LBMBPC), Department of Microbiology and Biochemistry, Faculty of Natural and Life Sciences, University Batna 2, Batna 05078, Algeria
Chawki Bensouici
Biotechnology Research Center, Ali Mendjli UV 03, Constantine 25000, Algeria
Nouicer Ferhat
Laboratory of Environment, Health and Animal Production (LESPA), Institute of Agriculture and Veterinary Sciences, University Batna 1, Batna 05000, Algeria
Mohamed Amine Kahoul
Laboratory of Food Sciences (LSA), Institute of Agriculture and Veterinary Sciences, University Batna 1, Batna 05000, Algeria
Mahmoud Helal
Department of Mechanical Engineering, Faculty of Engineering, Taif University, P.O. Box 11099, Taif 21944, Saudi Arabia
Rokayya Sami
Department of Food Science and Nutrition, College of Sciences, Taif University, P.O. Box 11099, Taif 21944, Saudi Arabia
Saif A. Alharthy
Department of Medical Laboratory Sciences, Faculty of Applied Medical Sciences, King Abdulaziz University, P.O. Box 80216, Jeddah 21589, Saudi Arabia
Roua S. Baty
Department of Biotechnology, College of Science, Taif University, P.O. Box 11099, Taif 21944, Saudi Arabia
Nouf H. Alsubhi
Biological Sciences Department, College of Science and Arts, King Abdulaziz University, Rabigh 21911, Saudi Arabia
Ghadeer I. Alrefaei
Department of Biology, College of Science, University of Jeddah, P.O. Box 80327, Jeddah 21589, Saudi Arabia
Abeer Elhakem
Department of Biology, College of Science and Humanities in Al-Kharj, Prince Sattam Bin Abdulaziz University, Al-Kharj 11942, Saudi Arabia
Sarah Alharthi
Department of Chemistry, College of Science, Taif University, P.O. Box 11099, Taif 21944, Saudi Arabia
Fahmy G. Elsaid
Biology Department, Science College, King Khalid University, P.O. Box. 960, Abha 61421, Saudi Arabia
Ali A. Shati
Biology Department, Science College, King Khalid University, P.O. Box. 960, Abha 61421, Saudi Arabia
The perennial aromatic plant Ruta tuberculata Forssk (Rutaceae) has been traditionally used by Mediterranean peoples as folk medicine against several types of disease to treat diverse illness. The objective of this work is to evaluate the in vitro and in vivo pharmacological activities of the aqueous (RAE) and methanolic (MeOH) 80% (RME) extracts of Algerian R. tuberculata aerial parts. Antioxidant potential, neuro-protective and anti-arthritic activities were investigated in vitro using six antioxidant approaches and by determining acetyl-cholinesterase and bovine albumin denaturation inhibitory capacities, respectively. Furthermore, in vivo anti-ulcer and anti-inflammatory activities were evaluated on EtOH-induced gastric mucosal damage and carrageenan-induced paw edema models in mice. Moreover, bio-compounds’ contents were also quantified using spectrophotometric and cLC-DAD methods. Both in vivo and in vitro investigations showed remarkable antioxidant activity of Ruta tuberculata Forssk, while methanolic extract (RME) of Ruta tuberculata Forssk exhibited more significant neuro-protective and anti-inflammatory effects. However, the antiulcer activity was more pronounced with RAE of R. tuberculata, which suggests that this plant can be considered as a natural resource of potent bioactive compounds that may act as antioxidant and anti-inflammatory agents, which underlines the importance of incorporating them in therapies in order to treat various diseases linked to oxidative stress, and they may also provide crucial data for the development of new anticholinesterase drugs to improve neurodegenerative diseases, such as Alzheimer’s.
