Corneal ulcer/keratitis derived Aspergillus flavus & Aspergillus tamarii and their RAPD-PCR typing

Bader Alshehri; Palanisamy Manikandan; Ahmed Abdel-hadi; Abdul Aziz Bin Dukhyil; Mohammed Alaidarous; Saeed Banawas; Raed Abdullah Alharbi; Saleh Abdullah Aloyuni; Csaba Vágvölgyi; László Kredics; Sándor Kocsubé; Esam S. Al-Malki; Rajendran Vijayakumar; Kanesan Panneer Selvam; Coimbatore Subramanian Shobana

Journal of King Saud University: Science (Apr 2020)

Corneal ulcer/keratitis derived Aspergillus flavus & Aspergillus tamarii and their RAPD-PCR typing

Bader Alshehri,
Palanisamy Manikandan,
Ahmed Abdel-hadi,
Abdul Aziz Bin Dukhyil,
Mohammed Alaidarous,
Saeed Banawas,
Raed Abdullah Alharbi,
Saleh Abdullah Aloyuni,
Csaba Vágvölgyi,
László Kredics,
Sándor Kocsubé,
Esam S. Al-Malki,
Rajendran Vijayakumar,
Kanesan Panneer Selvam,
Coimbatore Subramanian Shobana

Affiliations

Bader Alshehri: Department of Medical Laboratory Sciences, College of Applied Medical Sciences, Majmaah University, Al Majmaah 11952, Saudi Arabia; Health and Basic Sciences Research Center, Majmaah University, Majmaah 11952, Saudi Arabia
Palanisamy Manikandan: Department of Medical Laboratory Sciences, College of Applied Medical Sciences, Majmaah University, Al Majmaah 11952, Saudi Arabia; Greenlink Analytical and Research Laboratory India Private Limited, Coimbatore 641 014, India; Corresponding author at: Department of Medical Laboratory Sciences, College of Applied Medical Sciences, Majmaah University, Al Majmaah 11952, Saudi Arabia.
Ahmed Abdel-hadi: Department of Medical Laboratory Sciences, College of Applied Medical Sciences, Majmaah University, Al Majmaah 11952, Saudi Arabia; Botany and Microbiology Department, Faculty of Science, Al-Azhar University, Assiut Branch, Egypt
Abdul Aziz Bin Dukhyil: Department of Medical Laboratory Sciences, College of Applied Medical Sciences, Majmaah University, Al Majmaah 11952, Saudi Arabia
Mohammed Alaidarous: Department of Medical Laboratory Sciences, College of Applied Medical Sciences, Majmaah University, Al Majmaah 11952, Saudi Arabia; Health and Basic Sciences Research Center, Majmaah University, Majmaah 11952, Saudi Arabia
Saeed Banawas: Department of Medical Laboratory Sciences, College of Applied Medical Sciences, Majmaah University, Al Majmaah 11952, Saudi Arabia; Departments of Biomedical Sciences, Oregon State University, Corvallis, OR 97331, United States
Raed Abdullah Alharbi: Department of Public Health, College of Applied Medical Sciences, Majmaah University, Al Majmaah 11952, Saudi Arabia
Saleh Abdullah Aloyuni: Department of Public Health, College of Applied Medical Sciences, Majmaah University, Al Majmaah 11952, Saudi Arabia
Csaba Vágvölgyi: Department of Microbiology, Faculty of Science and Informatics, University of Szeged, Szeged, Hungary
László Kredics: Department of Microbiology, Faculty of Science and Informatics, University of Szeged, Szeged, Hungary
Sándor Kocsubé: Department of Microbiology, Faculty of Science and Informatics, University of Szeged, Szeged, Hungary
Esam S. Al-Malki: Department of Biology, College of Science in Zulfi, Majmaah University, Majmaah 11952, Saudi Arabia
Rajendran Vijayakumar: Department of Biology, College of Science in Zulfi, Majmaah University, Majmaah 11952, Saudi Arabia
Kanesan Panneer Selvam: Department of Microbiology, M.R Government Arts College, Mannargudi 614 001, India
Coimbatore Subramanian Shobana: Department of Microbiology, PSG College of Arts & Science, Coimbatore 641 014, India

Journal volume & issue: Vol. 32, no. 3
pp. 2103 – 2111

Abstract

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The aim of the study was investigate the DNA polymorphisms of Aspergillus flavus and Aspergillus tamarii isolated from regional fungal keratitis cases based on random amplified polymorphic DNA (RAPD) analyses. Thirty-two A. flavus and six A. tamarii isolates were identified using conventional macroscopic and microscopic features. Five decamer primers were used to amplify the genomic DNA sequences of the test isolates, and 140 total RAPD patterns were generated among them. From each of the 38 isolates, a range of 15–30 RAPD types were revealed. All of these were isolated from male farmers from Tamil Nadu. In the future, RAPD analyses could be employed as a reliable and rapid technique to differentiate among keratitis isolates of fungal pathogens. Keywords: RAPD PCR, Eye infection, Aspergillus flavus, Corneal ulcer, Molecular typing, Keratitis

Published in Journal of King Saud University: Science

ISSN: 1018-3647 (Print)
Publisher: Elsevier
Country of publisher: Saudi Arabia
LCC subjects: Science: Science (General)
Website: http://www.journals.elsevier.com/journal-of-king-saud-university-science/

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