Distinct Microbial Signatures Associated With Different Breast Cancer Types

Frontiers in Microbiology. 2018;9 DOI 10.3389/fmicb.2018.00951

 

Journal Homepage

Journal Title: Frontiers in Microbiology

ISSN: 1664-302X (Online)

Publisher: Frontiers Media S.A.

LCC Subject Category: Science: Microbiology

Country of publisher: Switzerland

Language of fulltext: English

Full-text formats available: PDF, HTML, ePUB, XML

 

AUTHORS

Sagarika Banerjee (Tumor Virology Program, Department of Otorhinolaryngology-Head and Neck Surgery and Microbiology, Abramson Cancer Center, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, United States)
Tian Tian (Department of Computer Science, New Jersey Institute of Technology, Newark, NJ, United States)
Zhi Wei (Department of Computer Science, New Jersey Institute of Technology, Newark, NJ, United States)
Natalie Shih (Department of Pathology and Laboratory Medicine, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, United States)
Michael D. Feldman (Department of Pathology and Laboratory Medicine, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, United States)
Kristen N. Peck (Tumor Virology Program, Department of Otorhinolaryngology-Head and Neck Surgery and Microbiology, Abramson Cancer Center, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, United States)
Angela M. DeMichele (Division of Hematology Oncology, Department of Medicine, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, United States)
James C. Alwine (Department of Cancer Biology, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, United States)
Erle S. Robertson (Tumor Virology Program, Department of Otorhinolaryngology-Head and Neck Surgery and Microbiology, Abramson Cancer Center, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, United States)

EDITORIAL INFORMATION

Blind peer review

Editorial Board

Instructions for authors

Time From Submission to Publication: 14 weeks

 

Abstract | Full Text

A dysbiotic microbiome can potentially contribute to the pathogenesis of many different diseases including cancer. Breast cancer is the second leading cause of cancer death in women. Thus, we investigated the diversity of the microbiome in the four major types of breast cancer: endocrine receptor (ER) positive, triple positive, Her2 positive and triple negative breast cancers. Using a whole genome and transcriptome amplification and a pan-pathogen microarray (PathoChip) strategy, we detected unique and common viral, bacterial, fungal and parasitic signatures for each of the breast cancer types. These were validated by PCR and Sanger sequencing. Hierarchical cluster analysis of the breast cancer samples, based on their detected microbial signatures, showed distinct patterns for the triple negative and triple positive samples, while the ER positive and Her2 positive samples shared similar microbial signatures. These signatures, unique or common to the different breast cancer types, provide a new line of investigation to gain further insights into prognosis, treatment strategies and clinical outcome, as well as better understanding of the role of the micro-organisms in the development and progression of breast cancer.