Animals (Apr 2021)

Assessment of <i>Lippia origanoides</i> Essential Oils in a <i>Salmonella typhimurium, Eimeria maxima,</i> and <i>Clostridium perfringens</i> Challenge Model to Induce Necrotic Enteritis in Broiler Chickens

  • Makenly E. Coles,
  • Aaron J. Forga,
  • Roberto Señas-Cuesta,
  • Brittany D. Graham,
  • Callie M. Selby,
  • Álvaro J. Uribe,
  • Blanca C. Martínez,
  • Jaime A. Angel-Isaza,
  • Christine N. Vuong,
  • Xochitl Hernandez-Velasco,
  • Billy M. Hargis,
  • Guillermo Tellez-Isaias

DOI
https://doi.org/10.3390/ani11041111
Journal volume & issue
Vol. 11, no. 1111
p. 1111

Abstract

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The objective of the present research was to evaluate dietary supplementation of essential oils from Lippia origanoides (LEO) on necrotic enteritis (NE). Chickens were randomly assigned to three groups. Group 1: negative control; Group 2: positive control challenged with Salmonella typhimurium (day 1), Eimeria maxima (day 18), and C. perfringens (CP, days 22-23); Group 3: dietary supplementation LEO and challenged. On d 25 of age, serum samples were collected to evaluate fluorescein isothiocyanate-dextran (FITC-d), superoxide dismutase (SOD), gamma interferon (IFN-γ), Immunoglobulin A (IgA). Group 3 showed a significant reduction of the harmful effects of induced infection/dysbiosis and a significant reduction in NE lesion scores, morbidity and mortality compared with the positive challenge control group (p p < 0.05). Group 3 also exhibited a significant reduction in FITC-d, IFN-γ and IgA compared with Group 2. However, a significant increase SOD was observed in Group 3 compared with both control groups. Further investigation to compare the effect of LEO and the standard treatment of clostridial NE is required.

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