Nature Communications (May 2018)

Rapid pathway prototyping and engineering using in vitro and in vivo synthetic genome SCRaMbLE-in methods

  • Wei Liu,
  • Zhouqing Luo,
  • Yun Wang,
  • Nhan T. Pham,
  • Laura Tuck,
  • Irene Pérez-Pi,
  • Longying Liu,
  • Yue Shen,
  • Chris French,
  • Manfred Auer,
  • Jon Marles-Wright,
  • Junbiao Dai,
  • Yizhi Cai

DOI
https://doi.org/10.1038/s41467-018-04254-0
Journal volume & issue
Vol. 9, no. 1
pp. 1 – 12

Abstract

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Pathway optimization and chassis engineering are usually carried out in a step-wise and trial-and-error manner. Here the authors present ’SCRaMbLE-in’ that combines in-vitro pathway rapid prototyping with in-vivo genome integration and optimization.