Data on calcium increases depending on stretch in dystrophic cardiomyocytes
E. Aguettaz,
J.J. Lopez,
A. Krzesiak,
B. Constantin,
C. Cognard,
S. Sebille
Affiliations
E. Aguettaz
Laboratoire de Signalisation et Transports Ioniques Membranaires (STIM), Equipe Transferts Ioniques et Rythmicité Cardiaque (TIRC), Université de Poitiers, 86073 Poitiers Cedex 9, France
J.J. Lopez
Laboratoire de Signalisation et Transports Ioniques Membranaires (STIM), Equipe Calcium et Microenvironnement des Cellules Souches (CMCS), Université de Poitiers, 86073 Poitiers Cedex 9, France
A. Krzesiak
Laboratoire de Signalisation et Transports Ioniques Membranaires (STIM), Equipe Transferts Ioniques et Rythmicité Cardiaque (TIRC), Université de Poitiers, 86073 Poitiers Cedex 9, France
B. Constantin
Laboratoire de Signalisation et Transports Ioniques Membranaires (STIM), Equipe Calcium et Microenvironnement des Cellules Souches (CMCS), Université de Poitiers, 86073 Poitiers Cedex 9, France
C. Cognard
Laboratoire de Signalisation et Transports Ioniques Membranaires (STIM), Equipe Transferts Ioniques et Rythmicité Cardiaque (TIRC), Université de Poitiers, 86073 Poitiers Cedex 9, France
S. Sebille
Laboratoire de Signalisation et Transports Ioniques Membranaires (STIM), Equipe Transferts Ioniques et Rythmicité Cardiaque (TIRC), Université de Poitiers, 86073 Poitiers Cedex 9, France; Corresponding author.
In this data article, intracellular Ca2+ concentration ([Ca2+]i) was measured in isolated ventricular Wild Type (WT) and mdx cardiomyocytes in two different conditions: at rest and during the application of an axial stretch. Using a carbon microfibers technique, axial stretch was applied to mimic effects of physiological conditions of ventricular filling. A study of cation entry with the same experimental model and the manganese quenching method reported (i) a constitutive cation entry in mdx cardiomyocytes and (ii) the involvement of TRPV2 channels in axial-stretch dependant cation entry, “Axial stretch-dependent cation entry in dystrophic cardiomyopathy: involvement of several TRPs channels” (Aguettaz et al., 2016) [1].Here, the Ca2+ dye fluo-8 was used for [Ca2+]i measurement, in both resting and stretching conditions, using a perfusion protocol starting initially with a calcium free Tyrode solution followed by the perfusion of 1.8 mM Ca2+ Tyrode solution. The variation of [Ca2+]i was found higher in mdx cardiomyocytes. Keywords: Calcium, TRPs channels, Stretch, Cardiomyocytes, Dystrophic
