Biocontrol effect of Bacillus amyloliquefaciens combined with un-irradiated and irradiated beet root as a preservative coating for pomegranate arils

Abstract

Pomegranate arils have medicial benefits and nutritional value, in order to preserve them and reduce spoilage during storage period. Biological control was applied by preserving coating composed from water and ethanolic extract of un-irradiated and irradiated beet root combined with isolated bacterial strain from pomegranate fruits (Bacillus amyloliquefaciens HAMO) under cold storage conditions. The obtained results of in vitro studies show that (irradiated beet root ethanolic extract/Bacillus amyloliquefaciens HAMO) preservative coating was the most effective treatment for the inhibition of undesirable microbial biomass but in vivo study (irradiated beet root water extract/Bacillus amyloliquefaciens HAMO) preservative coating gave better results than other treatments for postharvest trial. Irradiated beet root ethanolic extract/Bacillus amyloliquefaciens HAMO preservative coating gave the lowest weight loss percentage and higher total soluble solids percentage than other treatments. Pomegranate arils treated with (irradiated beet root water extract/Bacillus amyloliquefaciens HAMO) preservative coating scored higher titratable acidity percentage, total soluble phenols concentration, total soluble solids percentage and anthocyanin percentage than other treatments. Regarding total sugars percentage, D.D.P.H percentage and ascorbic acid concentration, data show that (irradiated beet root water extract/Bacillus amyloliquefaciens HAMO) and (irradiated beet root ethanolic extract/Bacillus amyloliquefaciens HAMO) preservative coatings maintain higher percentage and concentration of previous chemical constituents than other treatments.

Keywords

• Biocontrol
• Pomegranate arils
• Bacillus amyloliquefaciens
• Preservative coating
• Gamma irradiation