Glycolysis and gluconeogenesis are involved of glucose metabolism adaptation during fasting and re-feeding in black carp (Mylopharyngodon piceus)

Yafan Dai; Yubang Shen; Jiamin Guo; Hao Yang; Feng Chen; Wei Zhang; Wenhui Wu; Xiaoyan Xu; Jiale Li

Aquaculture and Fisheries (Mar 2024)

Glycolysis and gluconeogenesis are involved of glucose metabolism adaptation during fasting and re-feeding in black carp (Mylopharyngodon piceus)

Yafan Dai,
Yubang Shen,
Jiamin Guo,
Hao Yang,
Feng Chen,
Wei Zhang,
Wenhui Wu,
Xiaoyan Xu,
Jiale Li

Affiliations

Yafan Dai: Key Laboratory of Freshwater Aquatic Genetic Resources, Ministry of Agriculture and Rural Affairs, Shanghai Ocean University, Shanghai, 201306, China; Shanghai Engineering Research Centre of Aquaculture, Shanghai Ocean University, Shanghai, 201306, China
Yubang Shen: Key Laboratory of Freshwater Aquatic Genetic Resources, Ministry of Agriculture and Rural Affairs, Shanghai Ocean University, Shanghai, 201306, China; Shanghai Engineering Research Centre of Aquaculture, Shanghai Ocean University, Shanghai, 201306, China; Corresponding author. College of Aquaculture and Life science, Shanghai Ocean University, Shanghai, 201306, China.
Jiamin Guo: Key Laboratory of Freshwater Aquatic Genetic Resources, Ministry of Agriculture and Rural Affairs, Shanghai Ocean University, Shanghai, 201306, China; Shanghai Engineering Research Centre of Aquaculture, Shanghai Ocean University, Shanghai, 201306, China
Hao Yang: Key Laboratory of Freshwater Aquatic Genetic Resources, Ministry of Agriculture and Rural Affairs, Shanghai Ocean University, Shanghai, 201306, China; Shanghai Engineering Research Centre of Aquaculture, Shanghai Ocean University, Shanghai, 201306, China
Feng Chen: Key Laboratory of Freshwater Aquatic Genetic Resources, Ministry of Agriculture and Rural Affairs, Shanghai Ocean University, Shanghai, 201306, China; Shanghai Engineering Research Centre of Aquaculture, Shanghai Ocean University, Shanghai, 201306, China
Wei Zhang: Key Laboratory of Freshwater Aquatic Genetic Resources, Ministry of Agriculture and Rural Affairs, Shanghai Ocean University, Shanghai, 201306, China; Shanghai Engineering Research Centre of Aquaculture, Shanghai Ocean University, Shanghai, 201306, China
Wenhui Wu: Key Laboratory of Freshwater Aquatic Genetic Resources, Ministry of Agriculture and Rural Affairs, Shanghai Ocean University, Shanghai, 201306, China; Shanghai Engineering Research Centre of Aquaculture, Shanghai Ocean University, Shanghai, 201306, China
Xiaoyan Xu: Key Laboratory of Freshwater Aquatic Genetic Resources, Ministry of Agriculture and Rural Affairs, Shanghai Ocean University, Shanghai, 201306, China; Shanghai Engineering Research Centre of Aquaculture, Shanghai Ocean University, Shanghai, 201306, China
Jiale Li: Key Laboratory of Freshwater Aquatic Genetic Resources, Ministry of Agriculture and Rural Affairs, Shanghai Ocean University, Shanghai, 201306, China; Shanghai Engineering Research Centre of Aquaculture, Shanghai Ocean University, Shanghai, 201306, China; Corresponding author. College of Fisheries and Life Science, Shanghai Ocean University, 201306, Shanghai, China.

Journal volume & issue: Vol. 9, no. 2
pp. 226 – 233

Abstract

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Both in nature and in aquaculture, fish may experience periods of food scarcity or hunger. The metabolic regulation of fish when nutritional state changes is a complex process that involves many factors. To study glucose metabolism adaptability during fasting and re-feeding in the black carp (Mylopharyngodon piceus), we measured changes in some biochemical indicators related to glucose metabolism. Five fish were sampled on days 0, 1, 3, 5, and 10 of fasting (F, S1, S3, S5, and S10, respectively) and days 1, 3, and 5 of re-feeding (RF1, RF3, and RF5, respectively). The serum glucose concentration decreased significantly at S1, reached the lowest point at S10, and increased significantly at RF1 (P < 0.05). The concentration of liver glycogen decreased significantly at S1 and reached the lowest level at S3, whereas the muscle glycogen level decreased significantly at S5 and reached the lowest value at S10 (P < 0.05). Both liver and muscle glycogen levels returned to the pre-fasting level at RF5 (P < 0.05). Regarding glycolysis, the concentrations of pyruvate kinase (PK) and hexokinase (HK) decreased significantly at S5 and increased significantly at RF5 and RF1, respectively (P < 0.05). The concentrations of glucokinase (GCK) and insulin decreased significantly at S1 and increased significantly at RF1 and RF3, respectively (P < 0.05). The mRNA expression levels of liver GCK and glucose transporter 2 (GLUT2) decreased significantly at S1 and increased significantly at RF1 and RF5, respectively (P < 0.05). As for gluconeogenesis, the concentration of glucose-6-phosphatase (G6PC) increased significantly at S1 and decreased significantly at RF1 (P < 0.05). The concentrations of glucagon and glucocorticoid (GC) increased significantly at S3 and significantly decreased at RF1 and RF5, respectively (P < 0.05). The mRNA expression levels of liver G6PC and phosphoenolpyruvate carboxykinase (PEPCK) increased significantly at S3 and S1, and both decreased significantly at RF1 (P < 0.05). These results indicate that coordination between glycolysis and gluconeogenesis might be crucial for glucose homeostasis during fasting and re-feeding in the black carp.

Published in Aquaculture and Fisheries

ISSN: 2468-550X (Online)
Publisher: KeAi Communications Co., Ltd.
Country of publisher: China
LCC subjects: Agriculture: Aquaculture. Fisheries. Angling
Website: https://www.keaipublishing.com/en/journals/aquaculture-and-fisheries

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