Enhancement of Photosynthetic Iron-Use Efficiency Is an Important Trait of <i>Hordeum vulgare</i> for Adaptation of Photosystems to Iron Deficiency
Akihiro Saito,
Shotaro Shinjo,
Daiki Ito,
Yuko Doi,
Akira Sato,
Yuna Wakabayashi,
Juma Honda,
Yuka Arai,
Tsubasa Maeda,
Takuji Ohyama,
Kyoko Higuchi
Affiliations
Akihiro Saito
Laboratory of Biochemistry in Plant Productivity, Department of Agricultural Chemistry, Tokyo University of Agriculture, Setagaya-ku, Tokyo 156-8502, Japan
Shotaro Shinjo
Laboratory of Biochemistry in Plant Productivity, Department of Agricultural Chemistry, Tokyo University of Agriculture, Setagaya-ku, Tokyo 156-8502, Japan
Daiki Ito
Laboratory of Biochemistry in Plant Productivity, Department of Agricultural Chemistry, Tokyo University of Agriculture, Setagaya-ku, Tokyo 156-8502, Japan
Yuko Doi
Laboratory of Biochemistry in Plant Productivity, Department of Agricultural Chemistry, Tokyo University of Agriculture, Setagaya-ku, Tokyo 156-8502, Japan
Akira Sato
Laboratory of Biochemistry in Plant Productivity, Department of Agricultural Chemistry, Tokyo University of Agriculture, Setagaya-ku, Tokyo 156-8502, Japan
Yuna Wakabayashi
Laboratory of Biochemistry in Plant Productivity, Department of Agricultural Chemistry, Tokyo University of Agriculture, Setagaya-ku, Tokyo 156-8502, Japan
Juma Honda
Laboratory of Biochemistry in Plant Productivity, Department of Agricultural Chemistry, Tokyo University of Agriculture, Setagaya-ku, Tokyo 156-8502, Japan
Yuka Arai
Laboratory of Biochemistry in Plant Productivity, Department of Agricultural Chemistry, Tokyo University of Agriculture, Setagaya-ku, Tokyo 156-8502, Japan
Tsubasa Maeda
Laboratory of Biochemistry in Plant Productivity, Department of Agricultural Chemistry, Tokyo University of Agriculture, Setagaya-ku, Tokyo 156-8502, Japan
Takuji Ohyama
Laboratory of Biochemistry in Plant Productivity, Department of Agricultural Chemistry, Tokyo University of Agriculture, Setagaya-ku, Tokyo 156-8502, Japan
Kyoko Higuchi
Laboratory of Biochemistry in Plant Productivity, Department of Agricultural Chemistry, Tokyo University of Agriculture, Setagaya-ku, Tokyo 156-8502, Japan
Leaf iron (Fe) contents in Fe-deficiency-tolerant plants are not necessarily higher than that in Fe-deficiency-susceptible ones, suggesting an unknown mechanism involved in saving and allowing the efficient use of minimal Fe. To quantitatively evaluate the difference in Fe economy for photosynthesis, we compared the ratio of CO2 assimilation rate to Fe content in newly developed leaves as a novel index of photosynthetic iron-use efficiency (PIUE) among 23 different barley (Hordeum vulgare L.) varieties. Notably, varieties originating from areas with alkaline soil increased PIUE in response to Fe-deficiency, suggesting that PIUE enhancement is a crucial and genetically inherent trait for acclimation to Fe-deficient environments. Multivariate analyses revealed that the ability to increase PIUE was correlated with photochemical quenching (qP), which is a coefficient of light energy used in photosynthesis. Nevertheless, the maximal quantum yield of photosystem II (PSII) photochemistry, non-photochemical quenching, and quantum yield of carbon assimilation showed a relatively low correlation with PIUE. This result suggests that the ability of Fe-deficiency-tolerant varieties of barley to increase PIUE is related to optimizing the electron flow downstream of PSII, including cytochrome b6f and photosystem I.
