Using EuGeneCiD and EuGeneCiM computational tools for synthetic biology

Wheaton L. Schroeder; Anna S. Baber; Rajib Saha

STAR Protocols (Dec 2021)

Using EuGeneCiD and EuGeneCiM computational tools for synthetic biology

Wheaton L. Schroeder,
Anna S. Baber,
Rajib Saha

Affiliations

Wheaton L. Schroeder: Department of Chemical and Biomolecular Engineering, University of Nebraska – Lincoln, Lincoln, NE 68588, USA; Center for Root and Rhizobiome Innovation, University of Nebraska – Lincoln, Lincoln, NE 68588, USA; Corresponding author
Anna S. Baber: Center for Root and Rhizobiome Innovation, University of Nebraska – Lincoln, Lincoln, NE 68588, USA; Department of Biomedical Engineering, University of Rochester, Rochester, NY 14627, USA
Rajib Saha: Department of Chemical and Biomolecular Engineering, University of Nebraska – Lincoln, Lincoln, NE 68588, USA; Center for Root and Rhizobiome Innovation, University of Nebraska – Lincoln, Lincoln, NE 68588, USA; Corresponding author

Journal volume & issue: Vol. 2, no. 4
p. 100820

Abstract

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Summary: Synthetic biology often relies on the design of genetic circuits, utilizing “bioparts” (modular DNA pieces) to accomplish desired responses to external stimuli. While such designs are usually intuited, detailed here is a computational approach to synthetic biology design and modeling using optimization-based tools named Eukaryotic Genetic Circuit Design and Modeling. These allow for designing and subsequent screening of genetic circuits to increase the chances of in vivo success and contribute to the development of an application development pipeline.For complete details on the use and execution of this protocol, please refer to Schroeder, Baber, and Saha (2021).

Published in STAR Protocols

ISSN: 2666-1667 (Online)
Publisher: Elsevier
Country of publisher: United States
LCC subjects: Science: Science (General)
Website: https://star-protocols.cell.com/

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