Using mechanical homogenization to isolate microglia from mouse brain tissue to preserve transcriptomic integrity

Abstract

Summary: Numerous approaches have been developed to isolate microglia from the brain, but procedures using enzymatic dissociation at 37°C can introduce drastic transcriptomic changes and confound results from gene expression assays. Here, we present an optimized protocol for microglia isolation using mechanical homogenization. We use Dounce homogenization to homogenize mouse brain tissue into single-cell suspension. We then isolate microglia through Percoll gradient and flow cytometry. Isolated microglia exhibit a gene expression pattern without the changes induced by heated enzymatic digestion.For complete details on the use and execution of this protocol, please refer to Clayton et al. (2021). : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.

Keywords

• Classification Description: Cell biology
• Cell isolation
• Flow cytometry/Mass cytometry
• Cell separation/fractionation
• RNAseq
• Molecular biology