Histological assessment of pulpal responses to resin modified glass ionomer cements in human teeth

Abstract

Background: The biocompatibility of resin-modified glass ionomers (RMGIs) as a lining material is still under question. The present study evaluated the response of the pulp-dentin complex following application of resin-modified glass-ionomer cement, calcium hydroxide and conventional glass-ionomer in deep cavities prepared in human teeth. Materials and Methods: In this controlled clinical trial, 30 deep class V buccal cavities (3 mm × 2 mm × 2 mm) were prepared in human premolars treatment planned to be extracted for orthodontic reasons and divided into 3 groups. Groups were lined by a RMGI (Vivaglass), conventional glass Ionomer (Ionocid) and calcium hydroxide respectively. The cavities were subsequently filled with amalgam. Each group was then divided into two sub-groups according to time intervals 5 and 30 days. The patients were referred to Kerman Dental School and in accordance with orthodontic treatment plan; premolars were extracted and then prepared for histological assessment. The sections were stained with hematoxylin and eosin and periodic acid Schiff techniques. All of the samples were examined using a number of criteria including odontoblastic changes, inflammatory cells response, reactionary dentin formation and presence of microorganisms. The data were analyzed by Kruskal-Wallis and Mann-Whitney tests. P 0.05). Conclusion: Ionocid and Vivaglass resin-modified glass ionomers can be used as lining materials in human teeth.

Keywords

• Biocompatibility
• calcium hydroxide
• glass ionomer
• pulp response
• tertiary dentin