Encapsulation of human serum albumin in submicrometer magnetic poly(lactide-co-glycolide) particles as a model system for targeted drug delivery
Shubhra Quazi T.H.,
Macková Hana,
Horák Daniel,
Fodor-Kardos Andrea,
Tóth Judit,
Gyenis János,
Feczkó Tivadar
Affiliations
Shubhra Quazi T.H.
Research Institute of Chemical and Process Engineering, Doctoral School of Molecular and Nanotechnologies, Faculty of Information Technology, University of Pannonia, Egyetem u. 10, H-8200 Veszprém, Hungary
Macková Hana
Institute of Macromolecular Chemistry, Academy of Sciences of the Czech Republic, Heyrovsky Sq. 2, 162 06 Prague 6, Czech Republic
Horák Daniel
Institute of Macromolecular Chemistry, Academy of Sciences of the Czech Republic, Heyrovsky Sq. 2, 162 06 Prague 6, Czech Republic
Fodor-Kardos Andrea
Research Institute of Chemical and Process Engineering, Doctoral School of Molecular and Nanotechnologies, Faculty of Information Technology, University of Pannonia, Egyetem u. 10, H-8200 Veszprém, Hungary
Tóth Judit
Research Institute of Chemical and Process Engineering, Doctoral School of Molecular and Nanotechnologies, Faculty of Information Technology, University of Pannonia, Egyetem u. 10, H-8200 Veszprém, Hungary
Gyenis János
Research Institute of Chemical and Process Engineering, Doctoral School of Molecular and Nanotechnologies, Faculty of Information Technology, University of Pannonia, Egyetem u. 10, H-8200 Veszprém, Hungary
Feczkó Tivadar
Research Institute of Chemical and Process Engineering, Doctoral School of Molecular and Nanotechnologies, Faculty of Information Technology, University of Pannonia, Egyetem u. 10, H-8200 Veszprém, Hungary
Two types of iron oxide nanoparticles were synthesized by coprecipitation of Fe(II) and Fe(III) chlorides: water-dispersible γ-Fe2O3 and organic solvent-dispersible oleic acid-coated Fe3O4 particles. The nanoparticles, together with human serum albumin (HSA) serving as a model for a protein-type drug, were then incorporated in poly(lactide-co-glycolide) (PLGA) particles using double emulsion solvent evaporation technique. Morphology, size and particle size distribution of the resulting particles was analyzed by electron microscopy and dynamic light scattering. Iron oxide and HSA encapsulating efficiency was determined by Prussian Blue staining and micro-BCA assay, respectively.
