Pharmaceutical Sciences and Research (Apr 2018)
Evaluation of Propolis and Milk Administration on Caffein-Induced Mus musculus Fetus Skeletal
Abstract
Caffeine consumption by pregnant women at doses above 300 mg/ day cause skeletal damage. One solution to protect skeletal damage is propolis, propolis contains flavonoids, which could increase the number of osteoblasts. To compare the results obtained, used dairy sources of calcium and phosphorus which is very good for bone growth. In this research, testing to see the effect of propolis and milk on fetal skeletal female white mice (Mus musculus) induced by caffeine. Parent mice were divided into 6 groups. The negative control group, positive control group of caffeine (a dose of 75 mg/ kg weight), a positive control group of propolis (a dose of 1400 mg/ kg weight), a positive control group of milk (200 ml), D1 group (caffeine 75 mg / kg weight and propolis 1400 mg/ kg weight) and group D2 (caffeine 75 mg/ kg weight and milk 200 ml). Data were processed using one-way ANOVA. The results showed that administration of propolis and milk induced caffeine during pregnancy does not affect the parent body weight of mice, the number of fetuses and fetal weight was significantly (P> 0.05). Observations with Alizarin solution for groups D1 and D2 are not found skeletal defects as compared to the negative control group. It can be concluded that the provision of propolis dose of 1400 mg / kg and 200 ml of milk can repair skeletal damage caused by caffeine dose of 75 mg/ kg weight. The results showed that administration of propolis and milk induced caffeine during pregnancy does not affect the parent body weight of mice, the number of fetuses and fetal weight was significantly (P> 0.05). Observations with Alizarin solution for groups D1 and D2 are not found skeletal defects as compared to the negative control group. It can be concluded that the provision of propolis dose of 1400 mg/ kg weight and 200 ml of milk can repair skeletal damage caused by caffeine dose of 75 mg/ kg weight. The results showed that administration of propolis and milk induced caffeine during pregnancy does not affect the parent body weight of mice, the number of fetuses and fetal weight was significantly (P> 0.05). Observations with Alizarin solution for groups D1 and D2 are not found skeletal defects as compared to the negative control group. It can be concluded that the provision of propolis dose of 1400 mg/ kg weight and 200 ml of milk can repair skeletal damage caused by caffeine dose of 75 mg/ kg weight.
