Expression of miR-34a-5p and JAG1 in condyloma acuminatum tissues and their correlation with HPV typing and prognosis

Abstract

Objective To investigate the association of miR-34a-5p and JAG1 with type of human papillomavirus (HPV) and the prognosis. Methods Samples of condyloma acuminatum were collected from 101 patients with condyloma acuminatum (all HPV positive) at our hospital from March 2019 to March 2022. Samples were assigned to two groups, i.e., recurrence and non-recurrence according to whether recurrence occurred within 6 months after the treatments. Skin samples of either circumcision or vulvoplasty surgery from 98 individuals were used as the controls. The expression levels of miR-34a-5p and JAG1 mRNA were measured by real-time fluorescent quantitative PCR (qRT-PCR), while HPV genotyping test kit was used for HPV genotyping test. Pearson r was used to assess the correlation between miR-34a-5p and JAG1 mRNA levels in the patients with condyloma acuminatum. The ROC curve was used to determine the predictive value of miR-34a-5p and JAG1 mRNA levels for the recurrence of condyloma acuminatum. Results The expression levels of miR-34a-5p were lower, while JAG1 mRNA levels were higher in condyloma acuminatum than in the controls (P<0.05 for both). Expression levels of miR-34a-5p were negatively correlated with JAG1 mRNA levels in condyloma acuminatum tissues (r=-0.67, P<0.05). The expression levels of miR-34a-5p were lower in the wart body sized ≥10 mm diameter than in that sized <10 mm diameter (P<0.05). In contrast, expression levels of JAG1 mRNA were higher in the wart body sized≥ 10 mm diameter than in that sized <10 mm diameter (P<0.05). The expression levels of miR-34a-5p were lower, while JAG1 mRNA levels were higher in mixed condyloma acuminatum than in either high-risk or low-risk condyloma acuminatum (P<0.05). In comparison to non-recurrent condyloma acuminatum, the expression levels of miR-34a-5p were lower, while JAG1 mRNA levels were higher in recurrent condyloma acuminatum (P<0.05). For prediction of recurrence of condyloma acuminatum, the area under the curves of miR-34a-5p, JAG1 mRNA levels and their combination were 0.767, 0.821 and 0.891, respectively. Conclusions Condyloma acuminatum exhibits high expression levels of JAG1 mRNA and low levels of miR-34a-5p, which both are associated with types of HPV and prognosis.

Keywords

• condyloma acuminatum
• microrna-34a-5p
• jag1
• human papillomavirus typing
• prognosis