International Journal of Infectious Diseases (Oct 2021)
Serotype and genotype diversity of dengue viruses circulating in India: a multi-centre retrospective study involving the Virus Research Diagnostic Laboratory Network in 2018
- K. Alagarasu,
- J.A. Patil,
- M.B. Kakade,
- A.M. More,
- B. Yogesh,
- P. Newase,
- S.M. Jadhav,
- D. Parashar,
- H. Kaur,
- N. Gupta,
- N. Vijay,
- J. Narayan,
- P.S. Shah,
- Dr Ajanta Sharma,
- Dr Amita Jain,
- Dr Anudita Bhargava,
- Dr B. Anukumar,
- Dr Ashok M,
- Dr Bharti Malhotra,
- Dr B.J. Borkakoty,
- Dr Debasis Biswas,
- Dr SC Jaryal,
- Dr Kanwardeep Singh Dhingra,
- Dr K. Kaveri,
- Dr P.K. Khatri,
- Dr Manoj Kumar,
- Dr K. Nagamani,
- Dr Rahul Dhodapkar,
- Dr Rupinder Bakshi,
- Dr Paramjeet S. Gill,
- Dr Sanghamitra Pati,
- Dr Shanta Dutta,
- Dr Shantala G,
- Dr Usha Kalawat,
- Dr Disha A. Patel,
- Dr P. Vijayachari
Affiliations
- K. Alagarasu
- Dengue and Chikungunya Group, ICMR-National Institute of Virology, Pune, Maharashtra, India; Corresponding author. Address: Dengue/Chikungunya Group, ICMR-National Institute of Virology, Pune 411001, Maharashtra, India.
- J.A. Patil
- Dengue and Chikungunya Group, ICMR-National Institute of Virology, Pune, Maharashtra, India
- M.B. Kakade
- Dengue and Chikungunya Group, ICMR-National Institute of Virology, Pune, Maharashtra, India
- A.M. More
- Dengue and Chikungunya Group, ICMR-National Institute of Virology, Pune, Maharashtra, India
- B. Yogesh
- Dengue and Chikungunya Group, ICMR-National Institute of Virology, Pune, Maharashtra, India
- P. Newase
- Dengue and Chikungunya Group, ICMR-National Institute of Virology, Pune, Maharashtra, India
- S.M. Jadhav
- Dengue and Chikungunya Group, ICMR-National Institute of Virology, Pune, Maharashtra, India
- D. Parashar
- Dengue and Chikungunya Group, ICMR-National Institute of Virology, Pune, Maharashtra, India
- H. Kaur
- Virology Unit, Indian Council of Medical Research, New Delhi, India
- N. Gupta
- Virology Unit, Indian Council of Medical Research, New Delhi, India
- N. Vijay
- Virology Unit, Indian Council of Medical Research, New Delhi, India
- J. Narayan
- Virology Unit, Indian Council of Medical Research, New Delhi, India
- P.S. Shah
- Dengue and Chikungunya Group, ICMR-National Institute of Virology, Pune, Maharashtra, India
- Dr Ajanta Sharma
- Gauhati Medical College, Gauhati
- Dr Amita Jain
- King George Medical University, Lucknow
- Dr Anudita Bhargava
- All India Institute of Medical Sciences, Raipur
- Dr B. Anukumar
- ICMR-NIV Field Unit, Alapuzha
- Dr Ashok M
- ICMR-NIV Filed Unit, Bengaluru
- Dr Bharti Malhotra
- SMS Medical College, Jaipur
- Dr B.J. Borkakoty
- ICMR-Regional Research Medical Centre, Dibrugarh
- Dr Debasis Biswas
- All India Institute of Medical Sciences, Bhopal
- Dr SC Jaryal
- Dr. Rajendra Prasad Government Medical College, Tanda
- Dr Kanwardeep Singh Dhingra
- Government Medical College, Amritsar
- Dr K. Kaveri
- King Institute of Preventive Medicine & Research, Guindy, Chennai
- Dr P.K. Khatri
- SN Medical College, Jodhpur
- Dr Manoj Kumar
- Rajendra Institute of Medical Sciences, Ranchi
- Dr K. Nagamani
- Gandhi Medical College, Secunderabad
- Dr Rahul Dhodapkar
- Jawaharlal Nehru Institute of Postgraduate Medical Education and Research, Puducherry
- Dr Rupinder Bakshi
- Government Medical College, Patiala
- Dr Paramjeet S. Gill
- Post Graduate Institute of Medical Sciences, Rohtak
- Dr Sanghamitra Pati
- Regional Medical Research Centre, Bhubaneswar
- Dr Shanta Dutta
- ICMR-National Institute of Cholera & Enteric Diseases, Kolkata
- Dr Shantala G
- Bangalore Medical College & Research Institute, Bengaluru
- Dr Usha Kalawat
- Sri Venkateswara Institute of Medical Sciences, Tirupati
- Dr Disha A. Patel
- B.J. Medical College, Ahmedabad
- Dr P. Vijayachari
- ICMR-Regional Research Medical Centre, Port Blair
- Journal volume & issue
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Vol. 111
pp. 242 – 252
Abstract
ABSTRACT: Objectives: A retrospective study was undertaken to investigate the circulating dengue virus (DENV) serotypes and genotypes in India in 2018. Methods: In total, 4963 samples referred to virus research diagnostic laboratories (n=21), the Indian Council of Medical Research-National Institute of Virology (ICMR-NIV) and ICMR-NIV field units (n=2) for diagnosis of dengue in 2018 were tested using a real-time reverse transcription polymerase chain reaction assay for the presence of DENV serotypes. Representative samples were sequenced for the envelope (E) gene. Results: Regional diversity was observed with regard to the dominant circulating serotypes. DENV-2 was found to be the most common serotype in many states. Thrombocytopenia, petechiae and malaise were associated with DENV-2 infection. Phylogenetic analyses of DENV E gene sequences revealed the circulation of genotypes I and V of DENV-1, two lineages of DENV-2 genotype IV, DENV-3 genotype III and DENV-4 genotype I. Conclusions: This study found regional differences in the prevalence of circulating DENV serotypes in India, and provides baseline data for continuous molecular surveillance. Molecular surveillance may have implications for predicting large-scale outbreaks of dengue if regional shifts in the predominantly circulating serotypes and genotypes are detected during the early phase of the dengue season.
