Visualization of Hg2+ Stress on Plant Health at the Subcellular Level Revealed by a Highly Sensitive Fluorescent Sensor
Sumeera Asghar,
Zhenyang Yu,
Zheng Zhu,
Dengyue Zheng,
Zimo Zhao,
Yuming Xu,
Xiao Liu,
Chao Yuan,
Yan Li,
Wei Wang,
Jianfeng Xu,
Huailong Teng,
Jun Li,
Wen-Chao Yang,
Chunli Chen
Affiliations
Sumeera Asghar
State Key Laboratory of Green Pesticide, Key Laboratory of Green Pesticide and Agricultural Bioengineering, Ministry of Education,
Center for R&D of Fine Chemicals of Guizhou University, Guiyang 550025, China.
Zhenyang Yu
National Key Laboratory for Germplasm Innovation and Utilization for Fruit and Vegetable Horticultural Crops, Wuhan, China.
Zheng Zhu
Hubei Hongshan Laboratory, College of Life Science and Technology,
Huazhong Agricultural University, Wuhan, Hubei 430070, China.
Dengyue Zheng
Hubei Hongshan Laboratory, College of Life Science and Technology,
Huazhong Agricultural University, Wuhan, Hubei 430070, China.
Zimo Zhao
Hubei Hongshan Laboratory, College of Life Science and Technology,
Huazhong Agricultural University, Wuhan, Hubei 430070, China.
Yuming Xu
Hubei Hongshan Laboratory, College of Life Science and Technology,
Huazhong Agricultural University, Wuhan, Hubei 430070, China.
Xiao Liu
National Key Laboratory for Germplasm Innovation and Utilization for Fruit and Vegetable Horticultural Crops, Wuhan, China.
Chao Yuan
National Key Laboratory for Germplasm Innovation and Utilization for Fruit and Vegetable Horticultural Crops, Wuhan, China.
Yan Li
The Key Laboratory of Plant Resources Conservation Germplasm Innovation in Mountainous Region, College of Life Sciences, Institute of Agro-bioengineering,
Guizhou University, Guiyang 550025, China.
Wei Wang
State Key Laboratory of Green Pesticide, Key Laboratory of Green Pesticide and Agricultural Bioengineering, Ministry of Education,
Center for R&D of Fine Chemicals of Guizhou University, Guiyang 550025, China.
Jianfeng Xu
Hubei Hongshan Laboratory, College of Life Science and Technology,
Huazhong Agricultural University, Wuhan, Hubei 430070, China.
Huailong Teng
National Key Laboratory for Germplasm Innovation and Utilization for Fruit and Vegetable Horticultural Crops, Wuhan, China.
Jun Li
National Key Laboratory for Germplasm Innovation and Utilization for Fruit and Vegetable Horticultural Crops, Wuhan, China.
Wen-Chao Yang
State Key Laboratory of Green Pesticide, Key Laboratory of Green Pesticide and Agricultural Bioengineering, Ministry of Education,
Center for R&D of Fine Chemicals of Guizhou University, Guiyang 550025, China.
Chunli Chen
Hubei Hongshan Laboratory, College of Life Science and Technology,
Huazhong Agricultural University, Wuhan, Hubei 430070, China.
The presence of Hg2+ causes substantial stress to plants, adversely affecting growth and health by disrupting cell cycle divisions, photosynthesis, and ionic homeostasis. Accurate visualization of the spatiotemporal distribution of Hg2+ in plant tissues is crucial for the management of Hg pollution; however, the related research is still at its early stage. Herein, a small-molecule amphiphilic fluorescent probe (termed LJTP2) was developed for the specific detection of Hg2+ with a high sensitivity (~16 nM). Fluorescent imaging applications with LJTP2 not only detected the dynamic distribution of Hg2+ within plant cells at the subcellular level but also enabled the understanding of cell membrane health under Hg2+ stress. This study introduces a valuable imaging tool for elucidating the molecular mechanism of Hg2+ stress in plants, demonstrating the potential of the application of small-molecule fluorescent probes in plant science.
