Журнал органічної та фармацевтичної хімії (Oct 2023)

Metrological Characteristics of the Potentiometric Assay Developed for Determining the Antioxidant Activity of Ascorbic Acid

  • Oleksandr Yu. Maslov,
  • Sergii V. Kolisnyk,
  • Mykola A. Komisarenko,
  • Elshan Yu. Akhmedov,
  • Alla O. Koval,
  • Tatiana A. Kostina,
  • Olena V. Kolisnyk

DOI
https://doi.org/10.24959/ophcj.23.276394
Journal volume & issue
Vol. 21, no. 3
pp. 31 – 37

Abstract

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The potentiometric assay for determining the antioxidant activity of ascorbic acid has been developed and validated according to the following parameters: specificity, linearity, accuracy, repeatability, intermediate precision. The linearity was in the concentration range of 0.002 – 0.02 mol L–1 (r2 = 0.9993). The percentage of recovery was found to be in the range from 95.38 to 105.00 %. The values of %RSD for repeatability and intermediate precision were 1.86 and 1.95 %, respectively. The method is accurate and reliable, with the relative standard deviation of less than 2 %. It has been proven that the method developed is express, rapid, highly sensitive, accurate and sufficiently reliable.

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