EFEKTIFITAS MINYAK ATSIRI LENGKUAS TERHADAP PRODUKSI NITRIT OKSIDA MAKROFAG MENCIT

Abstract

Based on the content of the galangal's essential oil which can be used as antimicroorganism, analgesic, and antiseptic characterize with inhibiting and estructing microorgnism life process, it is predicted that there is a possibility of essential oil can be use for anti-inflammatory agent. Nitric-oxie (NO) is an unstable gas produced by cell such as macrophage and has the function as antimicroorganism. The aim of this study was to investigate whther essential oil of galangal components has an effect to the macrophage NO production, which stimulated by LPS E. coli.The both curative and preventive analysis using ANOVA showed that the NO productions differences were significant (p<0,01). The study showed that the NO levels produced by marine macrophages induced by LPS E. coli were suppressed by essential oil in a dose dependent fashion, suggesting antiinflammatory activities. Curatively, increased doses of the essential oil resulted in increased its anti-inflammatory functions. Five micro liter of the essential oil was preventively the most concentration as anti inflammation.

Keywords

• LPS
• Nitric-oxide (NO)
• elisa reader