Extraction of acid phosphomonoesterase from soil: testing of various extractants

Abstract

The aim of this work was to to investigate the suitability of various types of extractants for extraction of acid phosphomonoesterase from soil using various types of extractants. Succinate-borate buffer of pH 4.8 or 0.1 M K2SO4 were the most efficient to extract this enzyme compared to 0.1 M glutamic acid or 0.005 M salicylic acid. Extraction using 0.1 M glutamic acid gave significantly (P<0.05) lowest extraction yield. The following extracts were obtained: clear K2SO4 and glutamic acid extracts, succinate-borate buffer extracts were of slight coloration, and in some cases, rose-colored extracts of salicylic acid. The results of this work are in accordance with low extraction yields of phosphomonoesterase reported in other studies.

Keywords

• acid phosphomonoesterase
• extracts
• glutamic acid
• K₂SO₄
• salicylic acid
• soil