Data of indirect immunofluorescence labeling of the mouse brain sections with sera from SLE and MS patients
Ayse Ilksen Colpak,
Banu Balci-Peynircioglu,
Alp Can,
Yasemin Gursoy-Ozdemir,
Sevda Lule,
Umut Kalyoncu,
Turgay Dalkara
Affiliations
Ayse Ilksen Colpak
Department of Neurology, Faculty of Medicine, Hacettepe University, Ankara, Turkey
Banu Balci-Peynircioglu
Institute of Neurological Sciences and Psychiatry, Faculty of Medicine, Hacettepe University, Ankara, Turkey; Current affiliation: Department of Medical Biology, Faculty of Medicine, Hacettepe University, Ankara, Turkey
Alp Can
Department of Histology and Embryology, School of Medicine, Ankara University, Ankara, Turkey
Yasemin Gursoy-Ozdemir
Institute of Neurological Sciences and Psychiatry, Faculty of Medicine, Hacettepe University, Ankara, Turkey; Current affiliation: Department of Neurology, School of Medicine (KUSOM), Koç University, İstanbul, Turkey
Sevda Lule
Institute of Neurological Sciences and Psychiatry, Faculty of Medicine, Hacettepe University, Ankara, Turkey
Umut Kalyoncu
Department of Internal Medicine, Division of Rheumatology, Faculty of Medicine, Hacettepe University, Ankara, Turkey
Turgay Dalkara
Department of Neurology, Faculty of Medicine, Hacettepe University, Ankara, Turkey; Institute of Neurological Sciences and Psychiatry, Faculty of Medicine, Hacettepe University, Ankara, Turkey; Corresponding author at: Department of Neurology, Faculty of Medicine, Hacettepe University, Ankara, Turkey.
The data presented in this article are related to the research article entitled âBehcet Disease serum is immunoreactive to neurofilament medium which share common epitopes to bacterial HSP-65, a putative triggerâ (Lule et a. 2017) [1]. The immunoreactivity to self-antigens is well characterized for systemic lupus erythematosus (SLE) and multiple sclerosis (MS) (Magro Checa et al., 2013) [2]. Indirect immunofluorescence labeling of the mouse tissue sections with patient sera has recently been popular to discover novel epitopes and gain mechanistic insight to diseases with dysregulated immunity (Lennon et al., 2004) [3]. The present article demonstrates widespread labeling of cell nuclei with SLE patient sera and sporadic filamentous labeling along the axons with MS patient sera on mouse brain sections. The filamentous immunolabeling was sometimes associated with cytoplasmic staining of cells, which sent processes along the axon bundles, suggesting that they were oligodendrocytes. Since the mouse brain tissue has little autofluorescence and limited connective tissue causing non-specific immunolabeling, it appears superior to peripheral tissues for searching serum immunoreactivity. Keywords: Indirect immunofluorescence, Multiple sclerosis, Systemic lupus erythematosus, Autoimmunity
