POLYMORPHISM OF THE HAEMOSTASIS GENES IN WOMEN WITH THREATENED PRETERM DELIVERY

Abstract

Aim: to study polymorphism of the hemostasis-associated genes in women with physiological pregnancy and those with threatened preterm birth among the population of the Ivanovo region.Materials and methods. The study included 103 women with threatened preterm delivery and 73 women with physiological pregnancy. The women with threatened preterm delivery were divided into 2 subgroups: subgroup I totaled 53 women who had given birth in a timely manner; subgroup II consisted of 50 women who had had spontaneous birth before 37 weeks of gestation. In all patients, pregnancy was spontaneous and mono-fetal. The inclusion criteria for the main group were abdominal pain and structural changes in the cervix. Total genomic DNA was isolated from 100 µl of whole blood using a kit of «Trial-TOS-Genetics» («DNAtechnology», Russia). Single nucleotide polymorphisms (F2 G20210A, F5 G1691А, F7 G10976A, F13A1 G/T, FGB G -455A, PAI-1 -675 5G/4G, ITGA2 C807T, ITGB3 T1565C) were determined by polymerase chain reaction in real time using an iQS iCycler (Bio-Rad) machine and reagent kits of «Kardiogenetika. Thrombophilia» («DNA-technology», Russia).Results. In patients with threatened preterm delivery, the presence of polymorphic variants of the hemostasis-associated genes exceeds that in women with normal pregnancy. The observed accumulation of polymorphic genes may play a role in hemostasis dysfunction, which affects the blood fibrinolytic activity. As a result, the incidence of bleeding increases and the utero-placental circulation deteriorates.Conclusion. The obtained results indicate an adverse genetic background in part of women in this geographical region, which, in turn, might be caused by unfavorable environmental factors.

Keywords

• pregnancy
• threatened preterm delivery
• genotype
• polymorphic gene variants
• thrombophilia