Frontiers in Microbiology (Sep 2018)
Leishmania braziliensis Subverts Necroptosis by Modulating RIPK3 Expression
- Nivea F. Luz,
- Ricardo Khouri,
- Ricardo Khouri,
- Johan Van Weyenbergh,
- Johan Van Weyenbergh,
- Dalila L. Zanette,
- Dalila L. Zanette,
- Paloma P. Fiuza,
- Almerio Noronha,
- Aldina Barral,
- Aldina Barral,
- Viviane S. Boaventura,
- Viviane S. Boaventura,
- Deboraci B. Prates,
- Deboraci B. Prates,
- Francis Ka-Ming Chan,
- Bruno B. Andrade,
- Bruno B. Andrade,
- Bruno B. Andrade,
- Valeria M. Borges,
- Valeria M. Borges
Affiliations
- Nivea F. Luz
- Instituto Gonçalo Moniz, Oswaldo Cruz Foundation (FIOCRUZ), Salvador, Brazil
- Ricardo Khouri
- Instituto Gonçalo Moniz, Oswaldo Cruz Foundation (FIOCRUZ), Salvador, Brazil
- Ricardo Khouri
- Faculdade de Medicina da Bahia, Universidade Federal da Bahia, Salvador, Brazil
- Johan Van Weyenbergh
- Instituto Gonçalo Moniz, Oswaldo Cruz Foundation (FIOCRUZ), Salvador, Brazil
- Johan Van Weyenbergh
- Department of Microbiology and Immunology, Rega Institute for Medical Research, KU Leuven, Leuven, Belgium
- Dalila L. Zanette
- Instituto Gonçalo Moniz, Oswaldo Cruz Foundation (FIOCRUZ), Salvador, Brazil
- Dalila L. Zanette
- Faculdade de Medicina da Bahia, Universidade Federal da Bahia, Salvador, Brazil
- Paloma P. Fiuza
- Instituto Gonçalo Moniz, Oswaldo Cruz Foundation (FIOCRUZ), Salvador, Brazil
- Almerio Noronha
- Instituto Gonçalo Moniz, Oswaldo Cruz Foundation (FIOCRUZ), Salvador, Brazil
- Aldina Barral
- Instituto Gonçalo Moniz, Oswaldo Cruz Foundation (FIOCRUZ), Salvador, Brazil
- Aldina Barral
- Faculdade de Medicina da Bahia, Universidade Federal da Bahia, Salvador, Brazil
- Viviane S. Boaventura
- Instituto Gonçalo Moniz, Oswaldo Cruz Foundation (FIOCRUZ), Salvador, Brazil
- Viviane S. Boaventura
- Faculdade de Medicina da Bahia, Universidade Federal da Bahia, Salvador, Brazil
- Deboraci B. Prates
- Instituto Gonçalo Moniz, Oswaldo Cruz Foundation (FIOCRUZ), Salvador, Brazil
- Deboraci B. Prates
- Departamento de Biomorfologia, Instituto de Ciências da Saúde, Universidade Federal da Bahia, Salvador, Brazil
- Francis Ka-Ming Chan
- Department of Immunology, Duke University Medical Center, Durham, NC, United States
- Bruno B. Andrade
- Instituto Gonçalo Moniz, Oswaldo Cruz Foundation (FIOCRUZ), Salvador, Brazil
- Bruno B. Andrade
- Faculdade de Medicina da Bahia, Universidade Federal da Bahia, Salvador, Brazil
- Bruno B. Andrade
- Multinational Organization Network Sponsoring Translational and Epidemiological Research Initiative, Fundação José Silveira, Salvador, Brazil
- Valeria M. Borges
- Instituto Gonçalo Moniz, Oswaldo Cruz Foundation (FIOCRUZ), Salvador, Brazil
- Valeria M. Borges
- Faculdade de Medicina da Bahia, Universidade Federal da Bahia, Salvador, Brazil
- DOI
- https://doi.org/10.3389/fmicb.2018.02283
- Journal volume & issue
-
Vol. 9
Abstract
Leishmania braziliensis infection causes skin ulcers, typically found in localized cutaneous leishmaniasis (LCL). This tissue pathology associates with different modalities of cell necrosis, which are subverted by the parasite as a survival strategy. Herein we examined the participation of necroptosis, a specific form of programmed necrosis, in LCL lesions and found reduced RIPK3 and PGAM5 gene expression compared to normal skin. Assays using infected macrophages demonstrated that the parasite deactivates both RIPK3 and MLKL expression and that these molecules are important to control the intracellular L. braziliensis replication. Thus, LCL-related necroptosis may be targeted to control infection and disease immunopathology.
Keywords
