ANTIMICROBIAL AND CYTOTOXIC ACTIVITIES OF ALKALOIDS PRODUCED BY ENDOPHYTIC FUNGI

Abstract

In this study, 55 endophytic fungal strains isolated from alkaloid containing plants in Mongolia were screened for their production of alkaloids. TLC analysis of ethanolic crude extracts revealed the presence of alkaloids in 5 endophytic fungal strains. The alkaloid producing strains were identified based on their morphological characteristics and/or the D1/D2 domain sequences of 28S rRNA gene. The results showed that they all belong to the genus of Fusarium. Further, total alkaloid compounds of the 5 fungal strains were isolated and examined for their antimicrobial and cytotoxic activities. As results, alkaloid compounds of SFG-S1 and 16T6-F3 strains were active against yeast and fungi, and alkaloid compounds of SFG-R3, 16Т6-F3, 14R-1 strains exhibited cytotoxic activity against HepG2 liver cancer cells.

Keywords

• Endophytic fungi
• alkaloid
• antimicrobial activity
• cytotoxic activity