Учёные записки Санкт-Петербургского государственного медицинского университета им. Акад. И.П. Павлова (Dec 2016)

Development of murine model of allogeneic bone marrow transplantation and graft-versus-host disease

  • I. S. Moiseev,
  • E. A. Burmina,
  • A. V. Botina,
  • M. O. Popova,
  • A. R. Muslimov,
  • O. G. Smykova,
  • K. V. Lepik,
  • K. V. Melsitova,
  • A. I. Shakirova,
  • I. M. Barkhatov,
  • V. V. Baikov,
  • O. V. Galibin

DOI
https://doi.org/10.24884/1607-4181-2016-23-4-35-40
Journal volume & issue
Vol. 23, no. 4
pp. 35 – 40

Abstract

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Modeling of allogeneic bone marrow transplantation and graft-versus-host disease (GVHD) plays a crucial role in the improvement of transplantation procedures and testing novel immunosuppressive drugs. In this study we evaluated the feasibility of busulfan and cyclophosphamide conditioning with posttransplantation cyclophosphamide (150 mg/kg) in donorrecipient pairs C57Bl/6-Balb/с and Balb/с-C57Bl/6. We observed that Balb/с mice as opposed to C57Bl/6 mice can’t be the recipients because of the unacceptably high acute mortality from acute toxicity of chemotherapeutic drug. Histological studies in the C57Bl/6 recipients revealed acute GVHD in 93 % of animals. All animals had only grade I–II severity of GVHD. This model has the potential for future studies in the field of transplantology, nonetheless a decrease in the intensity of prophylaxis is required to augment the manifestations of GVHD.

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