Heliyon (Jul 2023)
Characterization of potential probiotics Lactobacillus species isolated from the gastrointestinal tract of Rhode Island Red (RIR) chicken in Ethiopia
Abstract
Background and aims: Nowadays, probiotic microorganisms are given high attention due to their potential for the improvement of animal production and productivity. The natural gut microflora of poultry can serve as an excellent source of optimum probiotic strains. Therefore, this study isolated, identified and characterized potential probiotic Lactobacillus strains from the digestive tract content of RIR chicken in Ethiopia. Methods: A total of five RIR chickens were randomly taken and a sample was taken from each gastrointestinal content for further analysis. For further characterization, among 190 isolates only 10 representative isolates were randomly taken for further in vitro probiotic potential characterization. The selected isolates were screened and identified based on the biochemical, morphological, and 16S rRNA gene sequences. Results: Survival isolates of IS3, IS4, IS6, and IS7 were significantly different (P < 0.05) at pH 2. IS3, IS4, IS6, and IS7 showed tolerance for 0.3% bile salt. Isolates of IS1, IS2, IS5, IS7, and IS8 were ampicillin-resistant, and chloramphenicol, ciprofloxacin, and erythromycin were used as antibiotics. All ten isolates showed antagonistic activity against Escherichia coli, Staphylococcus aureus, Salmonella typhimurium, Staphylococcus intermedius, and Salmonella enteritidis. The optimum temperature for all isolates was 45 °C, and all the isolates could grow at 0.69 mol/L of NaCl. Using phylogenetic analysis of the 16SrRNA gene sequence; IS3 was identified as Lactobacillus salivarius, while IS4, IS6, and IS7 were identified as Lactobacillus reuteri. Conclusions: The results indicated that the selected Lactobacillus isolates can survive the stress conditions of the gastrointestinal tract and can thus be considered potential probiotic candidates for chickens.
