Biochemical and behavioral responses in carp fish exposed to tricaine methane sulfonate (MS-222) as anesthetic drug under transport conditions

Abstract

This study aims to determine the effects of Tricaine methane‐sulfonate (MS-222) at concentration 150mg/l for one hour as an anesthetic agent to reduce the stress conditions during transfer the fish. Forty fish (Cyprinus carpio) were divide in to four groups: the 1st is control group (fish without both transfer and anesthesia), the 2nd group (fish transfer without anesthesia), the 3rd group (fish anesthesia without transfer) and the 4th group (fish transfer with anesthesia). The induction time reached to 3 minutes while the recovery time take at lasted for 7 to 10 minutes. Furthermore, the cortisol was significantly decreased in fish serum in both anesthetized groups that were transferred or that were left in the pond without transmission in compare to control groups (both transfer and without transfer fish). The glucose level rose significantly (P rd and 4th groups in compare to control groups. There was no difference in hematological parameters (hemoglobin and packed cell volume), blood sodium ion concentration, and enzyme activity represented by Alanine amino transferase in all groups while level of creatinine phosphate was decrease significantly in both groups 2nd and 3ed in compare to 1st group. It can be concluded that tricaine methane‐sulfonate stimulates the recovery and shortens the time of induction and reduces the stress condition caused by fish transport.

Keywords

• anesthetic drug
• stress
• fish transportation
• enzymatic and hematological study