A protocol for visualization of murine in situ neurovascular interfaces

Danielle D. Dang; Vikram Chandrashekhar; Vibhu Chandrashekhar; Nagela Ghabdanzanluqui; Russell H. Knutsen; Matthew A. Nazari; Likitha Nimmagadda; Danielle R. Donahue; Dorian B. McGavern; Beth A. Kozel; John D. Heiss; Karel Pacak; Zhengping Zhuang; Jared S. Rosenblum

STAR Protocols (Sep 2023)

A protocol for visualization of murine in situ neurovascular interfaces

Danielle D. Dang,
Vikram Chandrashekhar,
Vibhu Chandrashekhar,
Nagela Ghabdanzanluqui,
Russell H. Knutsen,
Matthew A. Nazari,
Likitha Nimmagadda,
Danielle R. Donahue,
Dorian B. McGavern,
Beth A. Kozel,
John D. Heiss,
Karel Pacak,
Zhengping Zhuang,
Jared S. Rosenblum

Affiliations

Danielle D. Dang: Neuro-Oncology Branch, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA; Surgical Neurology Branch, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, MD 20892, USA
Vikram Chandrashekhar: Neurosimplicity LLC, Shrewsbury, NJ 07702, USA
Vibhu Chandrashekhar: Neurosimplicity LLC, Shrewsbury, NJ 07702, USA
Nagela Ghabdanzanluqui: Viral Immunology and Intravital Imaging Section, National Institute of Neurological Disorders, National Institutes of Health, Bethesda, MD 20892, USA
Russell H. Knutsen: Laboratory of Vascular and Matrix Genetics, National Heart Lung and Blood Institute, National Institutes of Health, Bethesda, MD 20892, USA
Matthew A. Nazari: Eunice Kennedy Shriver National Institute of Child Health, Bethesda, MD 20892, USA
Likitha Nimmagadda: Laboratory of Vascular and Matrix Genetics, National Heart Lung and Blood Institute, National Institutes of Health, Bethesda, MD 20892, USA
Danielle R. Donahue: Mouse Imaging Facility, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, MD 20892, USA
Dorian B. McGavern: Viral Immunology and Intravital Imaging Section, National Institute of Neurological Disorders, National Institutes of Health, Bethesda, MD 20892, USA
Beth A. Kozel: Laboratory of Vascular and Matrix Genetics, National Heart Lung and Blood Institute, National Institutes of Health, Bethesda, MD 20892, USA
John D. Heiss: Surgical Neurology Branch, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, MD 20892, USA
Karel Pacak: Eunice Kennedy Shriver National Institute of Child Health, Bethesda, MD 20892, USA
Zhengping Zhuang: Neuro-Oncology Branch, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA; Corresponding author
Jared S. Rosenblum: Neurosimplicity LLC, Shrewsbury, NJ 07702, USA; Corresponding author

Journal volume & issue: Vol. 4, no. 3
p. 102367

Abstract

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Summary: Mapping cranial vasculature and adjacent neurovascular interfaces in their entirety will enhance our understanding of central nervous system function in any physiologic state. We present a workflow to visualize in situ murine vasculature and surrounding cranial structures using terminal polymer casting of vessels, iterative sample processing and image acquisition, and automated image registration and processing. While this method does not obtain dynamic imaging due to mouse sacrifice, these studies can be performed before sacrifice and processed with other acquired images.For complete details on the use and execution of this protocol, please refer to Rosenblum et al.1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.

Published in STAR Protocols

ISSN: 2666-1667 (Online)
Publisher: Elsevier
Country of publisher: United States
LCC subjects: Science: Science (General)
Website: https://star-protocols.cell.com/

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