Gaultheria procumbens essential oil longevity and oxidant/antioxidant status of silver catfish Rhamdia quelen experimentally infected by Aeromonas caviae
Camila Marina Verdi,
Matheus Dellaméa Baldissera,
Vanessa Schopf Machado,
Carine Freitas Milarch,
Eduardo Nascimento Correa de Andrade,
Gabrielle Aguiar Dantas,
Bernardo Baldisseroto,
Roberto Christ Vianna Santos
Affiliations
Camila Marina Verdi
Oral Microbiology Research Laboratory (LAPEMICRO), Federal University of Santa Maria, Santa Maria, Rio Grande do Sul, Brazil; Fish Physiology Laboratory (LAFIPE), Federal University of Santa Maria, Santa Maria, Rio Grande do Sul, Brazil
Matheus Dellaméa Baldissera
Laboratory of Bioprospecting and Experimental Biology, Franciscan University, Santa Maria, Rio Grande do Sul, Brazil; Fish Physiology Laboratory (LAFIPE), Federal University of Santa Maria, Santa Maria, Rio Grande do Sul, Brazil
Vanessa Schopf Machado
Oral Microbiology Research Laboratory (LAPEMICRO), Federal University of Santa Maria, Santa Maria, Rio Grande do Sul, Brazil; Fish Physiology Laboratory (LAFIPE), Federal University of Santa Maria, Santa Maria, Rio Grande do Sul, Brazil
Carine Freitas Milarch
Fish Physiology Laboratory (LAFIPE), Federal University of Santa Maria, Santa Maria, Rio Grande do Sul, Brazil; Superior Institute and Lutheran Educational Center Bom Jesus (IELUSC), Joinvile, Santa Catarina, Brazil
Eduardo Nascimento Correa de Andrade
Oral Microbiology Research Laboratory (LAPEMICRO), Federal University of Santa Maria, Santa Maria, Rio Grande do Sul, Brazil; Fish Physiology Laboratory (LAFIPE), Federal University of Santa Maria, Santa Maria, Rio Grande do Sul, Brazil
Gabrielle Aguiar Dantas
Oral Microbiology Research Laboratory (LAPEMICRO), Federal University of Santa Maria, Santa Maria, Rio Grande do Sul, Brazil; Fish Physiology Laboratory (LAFIPE), Federal University of Santa Maria, Santa Maria, Rio Grande do Sul, Brazil
Bernardo Baldisseroto
Laboratory of Bioprospecting and Experimental Biology, Franciscan University, Santa Maria, Rio Grande do Sul, Brazil; Fish Physiology Laboratory (LAFIPE), Federal University of Santa Maria, Santa Maria, Rio Grande do Sul, Brazil
Roberto Christ Vianna Santos
Oral Microbiology Research Laboratory (LAPEMICRO), Federal University of Santa Maria, Santa Maria, Rio Grande do Sul, Brazil; Fish Physiology Laboratory (LAFIPE), Federal University of Santa Maria, Santa Maria, Rio Grande do Sul, Brazil; Correspondence to: Faculdade Ielusc, Joinville, Santa Catarina, Brazil.
The increase in bacterial resistance in animals and the buildup of antimicrobials in animal meat pose significant nutritional and economic challenges. Given this context, this study explored the potential of Gaultheria procumbens essential oil (GPEO) as a sustainable alternative for aquaculture, focusing on its effects against Rhamdia quelen infected with Aeromonas caviae. Longevity tests revealed that R. quelen, when treated with 5 and 10 µL/L of GPEO, displayed significantly improved survival rates compared to both the untreated infected control group and those treated with ethanol. To understand the mechanisms behind the increased survival, a subsequent experiment with a 10 µL/L GPEO treatment aimed to investigate the oxidative stress pathway. This involved measuring levels of thiobarbituric acid reactive substances (TBARS) and reactive oxygen species (ROS), alongside enzyme activities for superoxide dismutase, glutathione peroxidase, and glutathione S-transferase. Our findings showed elevated TBARS and ROS levels in the non-infected control group treated with GPEO, suggesting an increase in these enzymes as a response to ROS balance. Therefore, our data indicate that the mechanism leading to enhanced longevity in R. quelen infected and treated with GPEO does not involve the oxidative stress pathway.