Frontiers in Chemistry (Apr 2019)

Long Wavelength TCF-Based Fluorescent Probe for the Detection of Alkaline Phosphatase in Live Cells

  • Lauren Gwynne,
  • Adam C. Sedgwick,
  • Jordan E. Gardiner,
  • George T. Williams,
  • Gyoungmi Kim,
  • John P. Lowe,
  • Jean-Yves Maillard,
  • A. Toby A. Jenkins,
  • Steven D. Bull,
  • Jonathan L. Sessler,
  • Juyoung Yoon,
  • Tony D. James

DOI
https://doi.org/10.3389/fchem.2019.00255
Journal volume & issue
Vol. 7

Abstract

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A long wavelength TCF-based fluorescent probe (TCF-ALP) was developed for the detection of alkaline phosphatase (ALP). ALP-mediated hydrolysis of the phosphate group of TCF-ALP resulted in a significant fluorescence “turn on” (58-fold), which was accompanied by a colorimetric response from yellow to purple. TCF-ALP was cell-permeable, which allowed it to be used to image ALP in HeLa cells. Upon addition of bone morphogenic protein 2, TCF-ALP proved capable of imaging endogenously stimulated ALP in myogenic murine C2C12 cells. Overall, TCF-ALP offers promise as an effective fluorescent/colorimetric probe for evaluating phosphatase activity in clinical assays or live cell systems.

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