Nature Communications (May 2021)
Selection of a picomolar antibody that targets CXCR2-mediated neutrophil activation and alleviates EAE symptoms
- Xiaojie Shi,
- Yue Wan,
- Nan Wang,
- Jiangchao Xiang,
- Tao Wang,
- Xiaofeng Yang,
- Ju Wang,
- Xuxue Dong,
- Liang Dong,
- Lei Yan,
- Yu Li,
- Lili Liu,
- Shinchen Hou,
- Zhenwei Zhong,
- Ian A. Wilson,
- Bei Yang,
- Guang Yang,
- Richard A. Lerner
Affiliations
- Xiaojie Shi
- Shanghai Institute for Advanced Immunochemical Studies, ShanghaiTech University
- Yue Wan
- Shanghai Institute for Advanced Immunochemical Studies, ShanghaiTech University
- Nan Wang
- Shanghai Institute for Advanced Immunochemical Studies, ShanghaiTech University
- Jiangchao Xiang
- Shanghai Institute for Advanced Immunochemical Studies, ShanghaiTech University
- Tao Wang
- Shanghai Institute for Advanced Immunochemical Studies, ShanghaiTech University
- Xiaofeng Yang
- Shanghai Institute for Advanced Immunochemical Studies, ShanghaiTech University
- Ju Wang
- Shanghai Institute for Advanced Immunochemical Studies, ShanghaiTech University
- Xuxue Dong
- Shanghai Institute for Advanced Immunochemical Studies, ShanghaiTech University
- Liang Dong
- Shanghai Institute for Advanced Immunochemical Studies, ShanghaiTech University
- Lei Yan
- Shanghai Institute for Advanced Immunochemical Studies, ShanghaiTech University
- Yu Li
- Shanghai Institute for Advanced Immunochemical Studies, ShanghaiTech University
- Lili Liu
- Shanghai Institute for Advanced Immunochemical Studies, ShanghaiTech University
- Shinchen Hou
- Shanghai Institute for Advanced Immunochemical Studies, ShanghaiTech University
- Zhenwei Zhong
- Shanghai Institute for Advanced Immunochemical Studies, ShanghaiTech University
- Ian A. Wilson
- Department of Integrative Structural and Computational Biology, The Scripps Research Institute, La Jolla
- Bei Yang
- Shanghai Institute for Advanced Immunochemical Studies, ShanghaiTech University
- Guang Yang
- Shanghai Institute for Advanced Immunochemical Studies, ShanghaiTech University
- Richard A. Lerner
- Shanghai Institute for Advanced Immunochemical Studies, ShanghaiTech University
- DOI
- https://doi.org/10.1038/s41467-021-22810-z
- Journal volume & issue
-
Vol. 12,
no. 1
pp. 1 – 14
Abstract
CXCR2 is central to neutrophil chemotaxis and hence to some inflammatory diseases. Here the authors demonstrate the value of an epitope-guided antibody panning method to develop a tight binding anti-hCXCR2 antibody, along with crystal structures of this antibody and antigen, that can block neutrophil chemotaxis and protect mice in an EAE model.
