Raman microscopy-based quantification of the physical properties of intracellular lipids

Abstract

Uematsu and Shimizu use Raman spectroscopy to quantify changes in the physical properties of lipid droplets (LDs) and in non-LD cell regions associated with alterations in saturated and unsaturated fatty acid availability in culture medium. Their results suggest that Raman spectroscopy can yield a quantitative measurement of changes in lipid conformation and can quantify trans / gauche ratios in lipid chains.