Optimization of Protease Production by Bacillus isronensis Strain KD3 Isolaed from Dairy Industry Effluent

Abstract

Proteases have a broad range of applications in pharmaceuticals, detergents and food processing industries. Protease producing strains are used profusely in industrial applications and the bioremediation process of wastewaters. In the present research work, efficient protease producing strain was isolated from dairy industry effluent. Screening of protease activity by isolates was checked by growing them on milk agar (skimmed) by spot inoculation method and further estimation was performed using quantitative protease assay. The efficient protease producing strain was identified based on morphological as well as biochemical characteristics as per standard keys of Bergey’s Manual of Determinative Bacteriology, later confirmed by 16s rRNA sequencing and BLAST analysis as Bacillus isronensis strain KD3. The maximum protease was produced at 42°C; pH 7-8; 200 rpm; and 7% inoculum concentration after 48h of the incubation period.

Keywords

• dairy industry effluent, protease activity, bacillus isronensis strain, kd3