A substrate-induced gating mechanism is conserved among Gram-positive IgA1 metalloproteases

Jasmina S. Redzic; Jeremy Rahkola; Norman Tran; Todd Holyoak; Eunjeong Lee; Antonio Javier Martín-Galiano; Nancy Meyer; Hongjin Zheng; Elan Eisenmesser

doi:10.1038/s42003-022-04173-3

Communications Biology (Nov 2022)

A substrate-induced gating mechanism is conserved among Gram-positive IgA1 metalloproteases

Jasmina S. Redzic,
Jeremy Rahkola,
Norman Tran,
Todd Holyoak,
Eunjeong Lee,
Antonio Javier Martín-Galiano,
Nancy Meyer,
Hongjin Zheng,
Elan Eisenmesser

Affiliations

Jasmina S. Redzic: Department of Biochemistry and Molecular Genetics, School of Medicine, University of Colorado Denver, School of Medicine
Jeremy Rahkola: Mucosal and Vaccine Research Program Colorado, Division of Infectious Disease, University of Colorado Denver School of Medicine and Denver Veterans Affairs Medical Center
Norman Tran: Department of Biology, University of Waterloo
Todd Holyoak: Department of Biology, University of Waterloo
Eunjeong Lee: Department of Biochemistry and Molecular Genetics, School of Medicine, University of Colorado Denver, School of Medicine
Antonio Javier Martín-Galiano: Core Scientific and Technical Units, Instituto de Salud Carlos III (ISCIII)
Nancy Meyer: Pacific Northwest Cryo-EM Center, Oregon Health and Science University
Hongjin Zheng: Department of Biochemistry and Molecular Genetics, School of Medicine, University of Colorado Denver, School of Medicine
Elan Eisenmesser: Department of Biochemistry and Molecular Genetics, School of Medicine, University of Colorado Denver, School of Medicine

DOI: https://doi.org/10.1038/s42003-022-04173-3
Journal volume & issue: Vol. 5, no. 1
pp. 1 – 10

Abstract

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Cryo-EM reveals the structure of the IgA1 metalloprotease (IgA1P) from the pathogen, G. haemolysans in the absence and presence of IgA substrate, which suggests a conserved gating mechanism for IgA1P enzymes and provides a basis for therapeutic strategies.

Published in Communications Biology

ISSN: 2399-3642 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science: Biology (General)
Website: https://www.nature.com/commsbio/

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