Acta Universitatis Agriculturae et Silviculturae Mendelianae Brunensis (Jan 2011)

In vitro testing of defense reactions in zygotic and somatic embryos of Abies numidica

  • Jiří Hřib,
  • Václav Adamec,
  • Božena Vooková

DOI
https://doi.org/10.11118/actaun201159060153
Journal volume & issue
Vol. 59, no. 6
pp. 153 – 160

Abstract

Read online

Defense of desiccated cotyledonary somatic embryos and mature zygotic embryos of Abies numidica was tested in vitro by dual cultures with tester, fungus Phaeolus schweinitzii. Both types of embryos expressed defense reactions manifested by inhibited growth of fungal tester towards the embryos. Mycelial growth was described by logistic sigmoid growth model with a single asymptote. Mutual comparisons of mycelial growth in presence of zygotic and somatic embryos showed significant differences in parameters of mycelium growth curves towards the embryos. Larger defense reactions were observed in zygotic embryos relative to somatic embryos and unlimited control cultivations without embryo. The possible role of auxin in the defense response of plant embryos is discussed.

Keywords