Cancers (Dec 2021)
Blood Bacterial DNA Load and Profiling Differ in Colorectal Cancer Patients Compared to Tumor-Free Controls
- Massimiliano Mutignani,
- Roberto Penagini,
- Giorgio Gargari,
- Simone Guglielmetti,
- Marcello Cintolo,
- Aldo Airoldi,
- Pierfrancesco Leone,
- Pietro Carnevali,
- Clorinda Ciafardini,
- Giulio Petrocelli,
- Federica Mascaretti,
- Barbara Oreggia,
- Lorenzo Dioscoridi,
- Federica Cavalcoli,
- Massimo Primignani,
- Francesco Pugliese,
- Paola Bertuccio,
- Pietro Soru,
- Carmelo Magistro,
- Giovanni Ferrari,
- Michela C. Speciani,
- Giulia Bonato,
- Marta Bini,
- Paolo Cantù,
- Flavio Caprioli,
- Marcello Vangeli,
- Edoardo Forti,
- Stefano Mazza,
- Giulia Tosetti,
- Rossella Bonzi,
- Maurizio Vecchi,
- Carlo La Vecchia,
- Marta Rossi
Affiliations
- Massimiliano Mutignani
- Digestive and Interventional Endoscopy Unit, ASST Grande Ospedale Metropolitano Niguarda, 20162 Milan, Italy
- Roberto Penagini
- Gastroenterology and Endoscopy Unit, Foundation IRCCS Ca’ Granda Ospedale Maggiore Policlinico, 20122 Milan, Italy
- Giorgio Gargari
- Department of Food, Environmental and Nutritional Sciences (DeFENS), University of Milan, 20133 Milan, Italy
- Simone Guglielmetti
- Department of Food, Environmental and Nutritional Sciences (DeFENS), University of Milan, 20133 Milan, Italy
- Marcello Cintolo
- Digestive and Interventional Endoscopy Unit, ASST Grande Ospedale Metropolitano Niguarda, 20162 Milan, Italy
- Aldo Airoldi
- Hepatology and Gastroenterology Unit, ASST Grande Ospedale Metropolitano Niguarda, 20162 Milan, Italy
- Pierfrancesco Leone
- General Surgery Unit, Foundation IRCCS Ca’ Granda Ospedale Maggiore Policlinico, 20122 Milan, Italy
- Pietro Carnevali
- Division of Minimally-Invasive Surgical Oncology, Niguarda Cancer Center, ASST Grande Ospedale Metropolitano Niguarda, 20162 Milan, Italy
- Clorinda Ciafardini
- Gastroenterology and Endoscopy Unit, Foundation IRCCS Ca’ Granda Ospedale Maggiore Policlinico, 20122 Milan, Italy
- Giulio Petrocelli
- Digestive and Interventional Endoscopy Unit, ASST Grande Ospedale Metropolitano Niguarda, 20162 Milan, Italy
- Federica Mascaretti
- Gastroenterology and Endoscopy Unit, Foundation IRCCS Ca’ Granda Ospedale Maggiore Policlinico, 20122 Milan, Italy
- Barbara Oreggia
- General Surgery Unit, Foundation IRCCS Ca’ Granda Ospedale Maggiore Policlinico, 20122 Milan, Italy
- Lorenzo Dioscoridi
- Digestive and Interventional Endoscopy Unit, ASST Grande Ospedale Metropolitano Niguarda, 20162 Milan, Italy
- Federica Cavalcoli
- Diagnostic and Therapeutic Endoscopy Unit, Fondazione IRCCS Istituto Nazionale Tumori, 20133 Milan, Italy
- Massimo Primignani
- Division of Gastroenterology and Hepatology, Foundation IRCCS Ca’ Granda Ospedale Maggiore Policlinico, 20122 Milan, Italy
- Francesco Pugliese
- Digestive and Interventional Endoscopy Unit, ASST Grande Ospedale Metropolitano Niguarda, 20162 Milan, Italy
- Paola Bertuccio
- Department of Clinical Science and Community Health, University of Milan, 20133 Milan, Italy
- Pietro Soru
- Division of Endoscopy, IRCCS Istituto Europeo di Oncologia, 20141 Milan, Italy
- Carmelo Magistro
- Division of Minimally-Invasive Surgical Oncology, Niguarda Cancer Center, ASST Grande Ospedale Metropolitano Niguarda, 20162 Milan, Italy
- Giovanni Ferrari
- Division of Minimally-Invasive Surgical Oncology, Niguarda Cancer Center, ASST Grande Ospedale Metropolitano Niguarda, 20162 Milan, Italy
- Michela C. Speciani
- Department of Clinical Science and Community Health, University of Milan, 20133 Milan, Italy
- Giulia Bonato
- Digestive and Interventional Endoscopy Unit, ASST Grande Ospedale Metropolitano Niguarda, 20162 Milan, Italy
- Marta Bini
- Digestive and Interventional Endoscopy Unit, ASST Grande Ospedale Metropolitano Niguarda, 20162 Milan, Italy
- Paolo Cantù
- Gastroenterology and Endoscopy Unit, Foundation IRCCS Ca’ Granda Ospedale Maggiore Policlinico, 20122 Milan, Italy
- Flavio Caprioli
- Gastroenterology and Endoscopy Unit, Foundation IRCCS Ca’ Granda Ospedale Maggiore Policlinico, 20122 Milan, Italy
- Marcello Vangeli
- Hepatology and Gastroenterology Unit, ASST Grande Ospedale Metropolitano Niguarda, 20162 Milan, Italy
- Edoardo Forti
- Digestive and Interventional Endoscopy Unit, ASST Grande Ospedale Metropolitano Niguarda, 20162 Milan, Italy
- Stefano Mazza
- Gastroenterology and Endoscopy Unit, Foundation IRCCS Ca’ Granda Ospedale Maggiore Policlinico, 20122 Milan, Italy
- Giulia Tosetti
- Division of Gastroenterology and Hepatology, Foundation IRCCS Ca’ Granda Ospedale Maggiore Policlinico, 20122 Milan, Italy
- Rossella Bonzi
- Department of Clinical Science and Community Health, University of Milan, 20133 Milan, Italy
- Maurizio Vecchi
- Gastroenterology and Endoscopy Unit, Foundation IRCCS Ca’ Granda Ospedale Maggiore Policlinico, 20122 Milan, Italy
- Carlo La Vecchia
- Department of Clinical Science and Community Health, University of Milan, 20133 Milan, Italy
- Marta Rossi
- Department of Clinical Science and Community Health, University of Milan, 20133 Milan, Italy
- DOI
- https://doi.org/10.3390/cancers13246363
- Journal volume & issue
-
Vol. 13,
no. 24
p. 6363
Abstract
Inflammation and immunity are linked to intestinal adenoma (IA) and colorectal cancer (CRC) development. The gut microbiota is associated with CRC risk. Epithelial barrier dysfunction can occur, possibly leading to increased intestinal permeability in CRC patients. We conducted a case-control study including 100 incident histologically confirmed CRC cases, and 100 IA and 100 healthy subjects, matched to cases by center, sex and age. We performed 16S rRNA gene analysis of blood and applied conditional logistic regression. Further analyses were based on negative binomial distribution normalization and Random Forest algorithm. We found an overrepresentation of blood 16S rRNA gene copies in colon cancer as compared to tumor-free controls. For high levels of gene copies, community diversity was higher in colon cancer cases than controls. Bacterial taxa and operational taxonomic unit abundances were different between groups and were able to predict CRC with an accuracy of 0.70. Our data support the hypothesis of a higher passage of bacteria from gastrointestinal tract to bloodstream in colon cancer. This result can be applied on non-invasive diagnostic tests for colon cancer control.
Keywords
- bacterial translocation
- bacterial 16S rRNA gene
- case-control study
- colon cancer diagnoses
- microbiome