Nature Communications (Jun 2018)

Immuno-detection by sequencing enables large-scale high-dimensional phenotyping in cells

  • Jessie A. G. van Buggenum,
  • Jan P. Gerlach,
  • Sabine E. J. Tanis,
  • Mark Hogeweg,
  • Pascal W. T. C. Jansen,
  • Jesse Middelwijk,
  • Ruud van der Steen,
  • Michiel Vermeulen,
  • Hendrik G. Stunnenberg,
  • Cornelis A. Albers,
  • Klaas W. Mulder

DOI
https://doi.org/10.1038/s41467-018-04761-0
Journal volume & issue
Vol. 9, no. 1
pp. 1 – 11

Abstract

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Detecting proteins and post-translational modifications is important for drug screens, but the number of proteins measurable simultaneously is limited. Here the authors use antibodies tagged with DNA barcodes and high-throughput sequencing to detect up to 70 (phospho-)proteins in stem cells.