Structural changes of Chlamydomonas reinhardtii cells during lipid enrichment and after solvent exposure
Sakina Bensalem,
Filipa Lopes,
Pierre Bodénès,
Dominique Pareau,
Olivier Français,
Bruno Le Pioufle
Affiliations
Sakina Bensalem
CNRS, SATIE, Ecole Normale Supérieure Paris Saclay, Université Paris-Saclay, 61 av du Pdt Wilson, 94230 Cachan, France; LGPM, EA 4038, CentraleSupélec, Université Paris Saclay, 3 Av. Joliot Curie, 91190 Gif-sur-Yvette, France
Filipa Lopes
LGPM, EA 4038, CentraleSupélec, Université Paris Saclay, 3 Av. Joliot Curie, 91190 Gif-sur-Yvette, France
Pierre Bodénès
CNRS, SATIE, Ecole Normale Supérieure Paris Saclay, Université Paris-Saclay, 61 av du Pdt Wilson, 94230 Cachan, France; LGPM, EA 4038, CentraleSupélec, Université Paris Saclay, 3 Av. Joliot Curie, 91190 Gif-sur-Yvette, France
Dominique Pareau
LGPM, EA 4038, CentraleSupélec, Université Paris Saclay, 3 Av. Joliot Curie, 91190 Gif-sur-Yvette, France
Olivier Français
ESIEE-Paris, ESYCOM EA 2552, Université Paris Est, 93160 Noisy Le Grand, France
Bruno Le Pioufle
CNRS, SATIE, Ecole Normale Supérieure Paris Saclay, Université Paris-Saclay, 61 av du Pdt Wilson, 94230 Cachan, France; Corresponding author.
Data are related to Confocal Laser Scanning Microscopy (CLSM) observations of lipid-enriched Chlamydomonas reinhardtii cells under different conditions. Firstly, the impact of stress conditions (nitrogen starvation) on the cell wall structure is assessed. Secondly is described the effect of solvents, in the context of lipid extraction, on the microalga's cell, particularly its lipid droplets, in the perspective of understanding the mechanisms behind solvent extraction of lipids. Furthermore, the role of the cell wall as a barrier to the solvent extraction action is highlighted.
